ArticleMolecular biology reports2025
Impact of soft tissue homogenization methods on RNA quality.
Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- Sequential Penta-Omic Extraction Method Using Single Biospecimens of Post-mortem Human Brain.bioRxiv : the preprint server for biology · 2026Article
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10 authors.
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Abstract
backgroundHigh-quality RNA isolation from tissues is crucial for transcriptomic analysis. The tissue disruption influences the RNA quality. The aim of this study was to compare different methods of tissue homogenization.
methodsThree homogenization methods were used (mortar and pestle, ball mill, and tissue homogenizer) to disrupt head and neck cancerous tissues, healthy tissues from free margin of head and neck cancer patients, and breast skin from breast cancer patients. The comparison of isolated RNA quantity and quality by measuring the concentration and absorbance, RIN, and Ct values of reference genes were performed.
resultsRNA isolated after tissue homogenizer usage has the highest 260/230 ratio (p = 0.02) and concentration (p = 0.02) also RIN values tend to be highest across all studied tissues. There are no significant differences between Ct values across all tissues processed with different homogenization methods; however, the Ct values of GAPDH and S18 are negatively correlated with RIN number (p = 0.002, p = 0.003).
conclusionThe tissue homogenizer is the most suitable for obtaining high-quality RNA across all examined tissues, which is essential in various RNA-based analyses. GAPDH and S18 Ct can indicate the RNA quality measured by RIN values.
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