ArticleBiochemistry and biophysics reports2025
Expressions of commonly used housekeeping proteins are altered in asthmatic mouse lungs.
Article in Biochemistry and biophysics reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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1 citing paper in PubMed.
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5 authors.
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Abstract
Housekeeping protein expression in tissue samples is often used to normalize immunoblotting data. However, utilizing common loading control proteins in pathological conditions without validation can be problematic. Here, we describe the alteration of commonly used loading control protein expressions in asthmatic lung samples and present a simpler and more reliable loading control for western blot analysis. Lung samples from control and asthmatic mice groups were used to assess the expression levels of three commonly used housekeeping proteins (beta-actin, alpha-tubulin, and glyceraldehyde-3-phosphate dehydrogenase). Ponceau S staining data was also obtained to assess the consistency of protein loading. Housekeeping protein expressions varied dramatically in asthmatic groups, ranging from 26 to 278 % compared to the control group (
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