Evidence map›Paper›PMID 40293710›Full record

ArticleHuman gene therapy2025

Bioinformatic Analysis of the Genetic Basis of Differential Adeno-Associated Virus Production Capability of 293 Variants.

Christopher R Herzog, Junping Zhang, Xiaomin Feng, Thao Thi Dang, Xiangping Yu, Jie Huang, Fang Fang, Hongyu Gao, Xuhong Yu, Yue Wang and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Human gene therapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Christopher R HerzogDepartment of Pediatrics, Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, Indiana, USA.
Junping ZhangDepartment of Pediatrics, Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, Indiana, USA.ORCID 0000-0001-9566-3875
Xiaomin FengGreater Bay Area Institute of Precision Medicine (Guangzhou), Fudan University, Guangzhou, China.
Thao Thi DangDepartment of Medical and Molecular Genetics, Indiana University, Indianapolis, Indiana, USA.
Xiangping YuDepartment of Pediatrics, Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, Indiana, USA.
Jie HuangDepartment of Pediatrics, Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, Indiana, USA.
Fang FangDepartment of Medical and Molecular Genetics, Indiana University, Indianapolis, Indiana, USA.
Hongyu GaoDepartment of Medical and Molecular Genetics, Indiana University, Indianapolis, Indiana, USA.
Xuhong YuDepartment of Medical and Molecular Genetics, Indiana University, Indianapolis, Indiana, USA.
Yue WangDepartment of Medical and Molecular Genetics, Indiana University, Indianapolis, Indiana, USA.
Renzhi HanDepartment of Pediatrics, Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, Indiana, USA.
Yulong LiuDepartment of Medical and Molecular Genetics, Indiana University, Indianapolis, Indiana, USA.
Kenneth CornettaDepartment of Medical and Molecular Genetics, Indiana University, Indianapolis, Indiana, USA.
Weidong XiaoDepartment of Pediatrics, Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, Indiana, USA.ORCID 0000-0002-9300-980X
Weihong XuGreater Bay Area Institute of Precision Medicine (Guangzhou), Fudan University, Guangzhou, China.

Funding

Toward Safer Gene Therapy for Hemophilia AP01HL160472 · NHLBI · INDIANA UNIVERSITY INDIANAPOLIS · PI Roland W. Herzog · 2022 to 2026
$15.1M
NHLBI NIH HHS P01 HL160472
6 · The paper itself

Abstract

Human embryonic kidney 293 (HEK 293) cells are the main producer cell line for recombinant adeno-associated virus (rAAV) production. However, AAV vector yields among 293 clones vary considerably. To elucidate the biological basis for these differences, whole genomes of an adherent and a suspension 293 cell clone with high-yield rAAV were sequenced using nanopore technology. All 293 cell derivative lines showed a twofold copy number gain at the adenoviral integration site across, suggesting a genome duplication event. To our surprise, the two high-producer clones, despite having been separately developed, are biologically closely grouped together as compared to other commonly used 293 clones. Their genomes contain a similar adenoviral gene integration region, which likely leads to high expression of proteins that facilitate AAV replication and packaging. Thus, genome duplication in the adenovirus integration locus may be a key factor affecting AAV production yield.

Indexed as

Computational BiologyDependovirusGenetic VectorsGenome, ViralHEK293 CellsHumansVirus IntegrationVirus Replication293 cellsgenetic basisvariants

Identifiers

PMID40293710
PMCPMC12171708

What Socratic holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.