Evidence map›Paper›PMID 40295870›Full record

ArticleNpj viruses2024

Synthetic recovery of Yada Yada virus expands insect-specific alphavirus knowledge and facilitates production of chimeric viruses.

Mikaela G Bell, Rhys H Parry, Tricia S E Lee, Gervais Habarugira, Isabella E McMahon, Madeline A Thompson, Naphak Modhiran, Daniel Watterson, Jana Batovska, Stacey E Lynch and 3 more

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In one paragraph

Article in Npj viruses, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors.

Mikaela G BellSchool of Chemistry and Molecular Biosciences, The University of Queensland St Lucia, Brisbane, Australia.
Rhys H ParrySchool of Chemistry and Molecular Biosciences, The University of Queensland St Lucia, Brisbane, Australia.
Tricia S E LeeSchool of Chemistry and Molecular Biosciences, The University of Queensland St Lucia, Brisbane, Australia.
Gervais HabarugiraSchool of Chemistry and Molecular Biosciences, The University of Queensland St Lucia, Brisbane, Australia.
Isabella E McMahonSchool of Chemistry and Molecular Biosciences, The University of Queensland St Lucia, Brisbane, Australia.
Madeline A ThompsonSchool of Chemistry and Molecular Biosciences, The University of Queensland St Lucia, Brisbane, Australia.
Naphak ModhiranSchool of Chemistry and Molecular Biosciences, The University of Queensland St Lucia, Brisbane, Australia.
Daniel WattersonSchool of Chemistry and Molecular Biosciences, The University of Queensland St Lucia, Brisbane, Australia.
Jana BatovskaAgriculture Victoria Research, AgriBio Centre for AgriBioscience, Bundoora, VIC, Australia.
Stacey E LynchAgriculture Victoria Research, AgriBio Centre for AgriBioscience, Bundoora, VIC, Australia.
Roy A HallSchool of Chemistry and Molecular Biosciences, The University of Queensland St Lucia, Brisbane, Australia.
Jessica J HarrisonSchool of Chemistry and Molecular Biosciences, The University of Queensland St Lucia, Brisbane, Australia. j.harrison1@uq.edu.au.
Jody Hobson-PetersSchool of Chemistry and Molecular Biosciences, The University of Queensland St Lucia, Brisbane, Australia. j.peters2@uq.edu.au.

Funding

Advance Queensland AQIRF067-2020-CV
6 · The paper itself

Abstract

Few insect-specific alphaviruses (ISA) have been discovered, with even fewer culturable to facilitate full characterisation. Here, we report the recovery of an infectious clone of Yada Yada virus (YYV)-a virus previously only detected by metagenomic sequencing of mosquito homogenates. Using the infectious clone, we confirmed the inability of YYV to replicate in vertebrate cells in vitro, with replication limited to only Aedes mosquito-derived cell lines. We further produced and characterised the first monoclonal antibodies (mAbs) to ISAs. Through successful replacement of the structural proteins of YYV with those of other ISAs, Eilat virus, Agua Salud (ASALV), Taï Forest (TALV) and Mwinilunga alphaviruses (MWAV), we established that a replication block for in vitro culture of TALV and MWAV in mosquito cells does not exist at virus entry. Unexpectedly, ASALV structural proteins were recognised by cross-reactive mAbs made to chikungunya (CHIKV) and Ross River viruses (RRV), suggesting a potential antigenic link between ASALV and pathogenic alphaviruses. The YYV genetic backbone was also investigated to generate chimeras displaying the structural proteins of various pathogenic vertebrate-infecting alphaviruses including CHIKV, RRV, Barmah Forest, Sindbis and Mayaro viruses. These chimeras retained the antigenic properties of the parental viruses and did not replicate in vertebrate cells, demonstrating the potential of the YYV platform for vaccine and diagnostic antigen production.

Identifiers

PMID40295870
PMCPMC11721364

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.