Evidence map›Paper›PMID 40299234›Full record

ArticleWorld journal of microbiology & biotechnology2025

Accelerated detection of carbapenem resistance in Klebsiella pneumoniae via single-cell Raman spectroscopy.

Jia-Cheng Ding, Li-Ting Dai, Jia-Xin Tang, Hui-Min Zhang, Le-Le Zhai, Chuang-Ye Cai, Zhi-Rou Xiao, Zhe-Mei Chen, Jia-Hui Ling, Meng-Xue Zou and 5 more

Abstract read
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In one paragraph

Article in World journal of microbiology & biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Jia-Cheng Ding *Microbiome Medicine Center, Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
Li-Ting Dai *Microbiome Medicine Center, Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
Jia-Xin TangDepartment of Clinical Laboratory, National Center for Respiratory Medicine, National Clinical Research Center for Respiratory Disease, State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Hui-Min ZhangMicrobiome Medicine Center, Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
Le-Le ZhaiQingdao Single-Cell Biotechnology, Co., Ltd, Qingdao, Shandong, China.
Chuang-Ye CaiMicrobiome Medicine Center, Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
Zhi-Rou XiaoDepartment of Clinical Laboratory, National Center for Respiratory Medicine, National Clinical Research Center for Respiratory Disease, State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Zhe-Mei ChenDepartment of Clinical Laboratory, National Center for Respiratory Medicine, National Clinical Research Center for Respiratory Disease, State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Jia-Hui LingMicrobiome Medicine Center, Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
Meng-Xue ZouMicrobiome Medicine Center, Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
Xi-Wu CaoMicrobiome Medicine Center, Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
Li-Juan LinMicrobiome Medicine Center, Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
Zi-Heng XuMicrobiome Medicine Center, Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
Pei-Bo YuanMicrobiome Medicine Center, Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China. pbyuanpb@163.com.
Ding-Qiang ChenMicrobiome Medicine Center, Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China. jyksys@126.com.

Funding

China Primary Health Care Foundation MTP2022D027Guangdong Province Science and Technology Innovation Strategy Special Fund 2019B020209001Natural Science Foundation of China 81974318Natural Science Foundation of China 82302588
6 · The paper itself

Abstract

Antibiotic resistance poses a significant global public health challenge, particularly concerning carbapenem-resistant Klebsiella pneumoniae (CRKP). A Raman-based antimicrobial susceptibility testing (R-AST) method was developed, utilizing D₂O-labeled single-cell Raman spectroscopy alongside the Metabolic Inhibition Level (MIL) for rapid CRKP antimicrobial susceptibility testing and carbapenemase enzyme typing. By analyzing 30,000 single-cell Raman spectra from 57 K. pneumoniae strains, the R-AST accurately detected CRKP within two hours. Furthermore, R-AST was able to differentiate between amikacin-sensitive and amikacin-resistant strains within the CRKP category, thereby providing potential treatment options. Notably, both R-AST classifications demonstrated 100% category agreement with the Vitek

Indexed as

Anti-Bacterial AgentsCarbapenem-Resistant EnterobacteriaceaeCarbapenemsKlebsiella pneumoniaeSingle-Cell AnalysisSpectrum Analysis, RamanAmikacinBacterial Proteinsbeta-LactamasesHumansKlebsiella InfectionsMicrobial Sensitivity TestsAmikacinAnti-Bacterial AgentsBacterial Proteinsbeta-LactamasescarbapenemaseCarbapenemsAntimicrobial susceptibility testingCarbapenemase enzyme typingCarbapenem resistanceKlebsiella pneumoniaeSingle-cell Raman spectroscopy

Identifiers

PMID40299234

What Socratic holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.