Evidence mapPaperPMID 40300855Full record

ArticleJournal for immunotherapy of cancer2025

Immunopeptidomics identified antigens for mRNA-lipid nanoparticle vaccines with alpha-galactosylceramide in multiple myeloma therapy.

Arne Van der Vreken, Fabien Thery, Chenggong Tu, Kevin Mwangi, Sofie Meulewaeter, Lien De Beck, Edith Janssens, Kim De Veirman, Karin Vanderkerken, Elke De Bruyne and 6 more

Abstract read
In one paragraph

Article in Journal for immunotherapy of cancer, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
  2. Chaperonin in health and disease.Molecular biomedicine · 2026
    Review
  3. Review
  4. Article
  5. Nanomedicine-Based Strategies for Multiple Myeloma Therapy.International journal of nanomedicine · 2026
    Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Arne Van der VrekenDepartment of Biomedical Sciences Brussels, Translational Oncology Research Center, Vrije Universiteit Brussel, Brussels, Belgium karine.breckpot@vub.be Eline.Menu@vub.be arne.van.der.vreken@vub.be.ORCID http://orcid.org/0000-0003-0345-721X
Fabien TheryVIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium.
Chenggong TuDepartment of Biomedical Sciences Brussels, Translational Oncology Research Center, Vrije Universiteit Brussel, Brussels, Belgium.
Kevin MwangiDepartment of Pharmaceutics, Laboratory of Physical Pharmacy and General Biochemistry, Ghent Research Group on Nanomedicines, Universiteit Gent, Ghent, Belgium.
Sofie MeulewaeterDepartment of Pharmaceutics, Laboratory of Physical Pharmacy and General Biochemistry, Ghent Research Group on Nanomedicines, Universiteit Gent, Ghent, Belgium.
Lien De BeckDepartment of Biomedical Sciences Brussels, Translational Oncology Research Center, Vrije Universiteit Brussel, Brussels, Belgium.
Edith JanssensDepartment of Biomedical Sciences Brussels, Translational Oncology Research Center, Vrije Universiteit Brussel, Brussels, Belgium.
Kim De VeirmanDepartment of Biomedical Sciences Brussels, Translational Oncology Research Center, Vrije Universiteit Brussel, Brussels, Belgium.ORCID http://orcid.org/0000-0002-1313-6121
Karin VanderkerkenDepartment of Biomedical Sciences Brussels, Translational Oncology Research Center, Vrije Universiteit Brussel, Brussels, Belgium.
Elke De BruyneDepartment of Biomedical Sciences Brussels, Translational Oncology Research Center, Vrije Universiteit Brussel, Brussels, Belgium.
Lorenzo FranceschiniDepartment of Biomedical Sciences Brussels, Translational Oncology Research Center, Vrije Universiteit Brussel, Brussels, Belgium.
Francis ImpensVIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium.
Rein VerbekeDepartment of Pharmaceutics, Laboratory of Physical Pharmacy and General Biochemistry, Ghent Research Group on Nanomedicines, Universiteit Gent, Ghent, Belgium.
Ine LentackerDepartment of Pharmaceutics, Laboratory of Physical Pharmacy and General Biochemistry, Ghent Research Group on Nanomedicines, Universiteit Gent, Ghent, Belgium.
Eline MenuDepartment of Biomedical Sciences Brussels, Translational Oncology Research Center, Vrije Universiteit Brussel, Brussels, Belgium karine.breckpot@vub.be Eline.Menu@vub.be arne.van.der.vreken@vub.be.
Karine BreckpotDepartment of Biomedical Sciences Brussels, Translational Oncology Research Center, Vrije Universiteit Brussel, Brussels, Belgium karine.breckpot@vub.be Eline.Menu@vub.be arne.van.der.vreken@vub.be.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundInvariant natural killer T (iNKT) cells and CD8

methodsMicroarray data and immunopeptidomics (imPep) were used to identify candidate antigens for immunization in 5TMM models. Galsomes, lipid nanoparticles containing antigen mRNA and αGC were used to immunize 5T33MM-bearing mice. This treatment was combined with a CD40 agonist. Tumor burden and activation of iNKT cells and CD8

resultsRNA transcripts revealed survivin as a candidate antigen. Prime-boost Galsomes therapy targeting survivin significantly reduced M-protein levels despite low survivin-specific T cell responses. Further analysis showed potential T cell fratricide. ImPep revealed HSP60, Idiotype, PICALM and EF1A1 as candidate antigens. Prime-boost therapy with Galsomes targeting these antigens reduced MM growth significantly when combined with a CD40 agonist, coinciding with significantly improved antigen presentation, costimulation and cytotoxicity of iNKT cells and CD8

conclusionThese findings highlight the potential of Galsomes, an mRNA vaccine designed to activate CD8

Indexed as

Antigens, NeoplasmCancer VaccinesGalactosylceramidesMultiple MyelomaNanoparticlesRNA, MessengerAnimalsCD8-Positive T-LymphocytesCell Line, TumorFemaleHumansLiposomesMiceNanovaccinesNatural Killer T-Cellsalpha-galactosylceramideAntigens, NeoplasmCancer VaccinesGalactosylceramidesLipid NanoparticlesLiposomesNanovaccinesRNA, MessengerImmunotherapyMajor histocompatibility complex - MHCMultiple MyelomaNanoparticleVaccine

Identifiers

PMID40300855
PMCPMC12049997

What Socratic holds

Textmetadata
LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.