Evidence map›Paper›PMID 40304913›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

ATR-Infrared Spectroscopy and Acoustic Sensing in Characterization of Blood and Pancreatic Ductal Adenocarcinoma-Derived Extracellular Vesicles.

Imola Cs Szigyártó, Priyanka Singh, Tasvilla Sonallya, Loránd Románszki, Judith Mihály, Zoltán Wiener, Tímea Bebesi, Gábor Mészáros, Zsófia Keresztes, Michael Thompson and 2 more

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Imola Cs SzigyártóHUN-REN Research Centre for Natural Sciences, Institute of Materials and Environmental Chemistry, Biomolecular Self-assembly Research Group, Budapest, Hungary. szigyarto.imola.csilla@ttk.hu.
Priyanka SinghHUN-REN Research Centre for Natural Sciences, Institute of Materials and Environmental Chemistry, Biomolecular Self-assembly Research Group, Budapest, Hungary.
Tasvilla SonallyaHUN-REN Research Centre for Natural Sciences, Institute of Materials and Environmental Chemistry, Biomolecular Self-assembly Research Group, Budapest, Hungary.
Loránd RománszkiHUN-REN Research Centre for Natural Sciences, Institute of Materials and Environmental Chemistry, Functional Interfaces Research Group, Budapest, Hungary.
Judith MihályHUN-REN Research Centre for Natural Sciences, Institute of Materials and Environmental Chemistry, Biological Nanochemistry Research Group, Budapest, Hungary.
Zoltán WienerDepartment of Genetics, Cell and Immunobiology, Semmelweis University, Budapest, Hungary.
Tímea BebesiHevesy György PhD School of Chemistry, Eötvös Loránd University, Budapest, Hungary.
Gábor MészárosHUN-REN Research Centre for Natural Sciences, Institute of Materials and Environmental Chemistry, Functional Interfaces Research Group, Budapest, Hungary.
Zsófia KeresztesHUN-REN Research Centre for Natural Sciences, Institute of Materials and Environmental Chemistry, Functional Interfaces Research Group, Budapest, Hungary.
Michael ThompsonDepartment of Chemistry, University of Toronto, Toronto, ON, Canada.
Zoltán VargaHUN-REN Research Centre for Natural Sciences, Institute of Materials and Environmental Chemistry, Biological Nanochemistry Research Group, Budapest, Hungary.
Tamás Beke-SomfaiHUN-REN Research Centre for Natural Sciences, Institute of Materials and Environmental Chemistry, Biomolecular Self-assembly Research Group, Budapest, Hungary. beke-somfai.tamas@ttk.hu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Extracellular vesicles (EVs) are phospholipid bilayer-enclosed nanoparticles secreted by most cells into the biofluids. They have an important role in intercellular communication by carrying bioactive components (e.g., lipids, proteins, nucleic acids, and other metabolites) and reflecting the biochemical and metabolic processes of their parent cell. Due to their widespread molecular cargo, they have potential diagnostic and therapeutic roles, so they can be considered as new generation biomarkers. With the drastic increase in the number of publications and the comparability of the obtained results, the development of standardized protocols has become necessary. Here we aim to demonstrate the applicability of two, non-conventional techniques, such as ATR-infrared spectroscopy (ATR-FTIR) and electromagnetic piezoelectric acoustic sensor (EMPAS) in the characterization of compositional changes of blood- and pancreatic tumor-derived vesicles. In addition, we provide guidelines in sample collection, isolation, and separation of EVs. IR spectroscopy, as a fast and label-free technique, gives biochemical insight into the sample composition, while EMPAS serves information on vesicle quantity and in membrane integrity changes during storage.

Indexed as

Carcinoma, Pancreatic DuctalExtracellular VesiclesPancreatic NeoplasmsAcousticsHumansSpectroscopy, Fourier Transform InfraredElectromagnetic piezoelectric acoustic sensorExtracellular vesiclesInfrared spectroscopyIsolationPancreatic cell linePlateletRed blood cellSize-exclusion chromatography

Identifiers

PMID40304913

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.