High Content Analysis of Mitochondrial Function in Induced Pluripotent Stem Cell-Derived Neurons.

Daniel Little, Christin Luft, Olukunbi Mosaku, Robin Ketteler, Michael J Devine, Paul Gissen

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Daniel LittleUniversity College London, London, UK.

Christin LuftUniversity College London, London, UK.

Olukunbi MosakuUniversity College London, London, UK.

Robin KettelerUniversity College London, London, UK.ORCID https://orcid.org/0000-0002-2786-7291

Michael J DevineUniversity College London, London, UK.ORCID https://orcid.org/0000-0001-6076-3382

Paul GissenUniversity College London, London, UK. p.gissen@ucl.ac.uk.ORCID https://orcid.org/0000-0002-9712-6122

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mitochondrial dysfunction is linked to many neurological diseases; therefore, the ability to measure mitochondrial function is of great use for researching disease and testing potential therapeutics. Here we describe a high-content assay to simultaneously measure mitochondrial membrane potential, morphology, and cell viability in iPSC-derived neurons. Neurons are seeded into plates suitable for fluorescent microscopy, and stained with the mitochondrial membrane potential-dependent dye TMRM, cytoplasmic dye Calcein-AM, and nuclear stain Hoechst-33,342. Images are acquired in live cells and analyzed using automated image analysis software.

Indexed as

Induced Pluripotent Stem CellsMitochondriaNeuronsCell DifferentiationCells, CulturedCell SurvivalHumansMembrane Potential, MitochondrialMicroscopy, FluorescenceHigh content screeningImage analysisInduced pluripotent stem cellsMitochondriaNeurons

Identifiers

PMID40307649

DOI10.1007/978-1-0716-4530-7_19

What Socratic holds

Textmetadata

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.