Evidence map›Paper›PMID 40309301›Full record

ArticleBiophysics and physicobiology2025

Time-resolved small-angle X-ray scattering system development for the biological macromolecules at SACLA: A pilot study.

Nobutaka Shimizu, Fangjia Luo, Tomoyuki Tanaka, Kensuke Tono, Keiko Yatabe, So Iwata, Eriko Nango

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biophysics and physicobiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Nobutaka ShimizuRIKEN SPring-8 Center, Sayo, Hyogo 679-5148, Japan.
Fangjia LuoJapan Synchrotron Radiation Research Institute, Sayo, Hyogo 679-5198, Japan.
Tomoyuki TanakaDepartment of Cell Biology, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan.
Kensuke TonoRIKEN SPring-8 Center, Sayo, Hyogo 679-5148, Japan.
Keiko YatabePhoton Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK), Tsukuba, Ibaraki 305-0801, Japan.
So IwataDepartment of Cell Biology, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan.
Eriko NangoRIKEN SPring-8 Center, Sayo, Hyogo 679-5148, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A time-resolved small-angle X-ray scattering (SAXS) system for protein solution samples using an X-ray free-electron laser (XFEL) was established by developing a SAXS diffractometer by integrating a helium path into the DAPHNIS system initially designed for Serial Femtosecond Crystallography (SFX) experiments at BL2 of SACLA. This modification enabled us to successfully capture the SAXS profiles of ovalbumin under conditions without any reaction trigger, using both the newly developed system and the sample solution flow device that was originally designed for SFX experiments. Furthermore, we conducted acid denaturation experiments on cytochrome c, using a T-junction-type solution mixing flow system, and observed the denaturation-induced changes in the SAXS profiles.

Indexed as

BioSAXSsolution X-ray scatteringtime-resolved measurementXFEL

Identifiers

PMID40309301
PMCPMC12040570

What Socratic holds

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LicenceCC BY-NC-SA
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.