Evidence map›Paper›PMID 40309791›Full record

ArticleBrain sciences2025

Ethanol Administration in Mice Leads to Sex-Specific Changes in the Acetylation of α-Tubulin in the Cerebellum.

Abosede Elesinnla, Rehana Khatoon, Nicholas Kleinert, Junfang Wu, Jaylyn Waddell, Tibor Kristian

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Brain sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Abosede ElesinnlaDepartment of Anesthesiology and the Center for Shock, Trauma and Anesthesiology (S.T.A.R.), University of Maryland School of Medicine, 685 Baltimore Street, Baltimore, MD 21201, USA.
Rehana KhatoonDepartment of Anesthesiology and the Center for Shock, Trauma and Anesthesiology (S.T.A.R.), University of Maryland School of Medicine, 685 Baltimore Street, Baltimore, MD 21201, USA.
Nicholas KleinertVeterans Affairs Maryland Health Center System, 10 North Greene Street, Baltimore, MD 21201, USA.
Junfang WuDepartment of Anesthesiology and the Center for Shock, Trauma and Anesthesiology (S.T.A.R.), University of Maryland School of Medicine, 685 Baltimore Street, Baltimore, MD 21201, USA.ORCID 0000-0003-3338-7291
Jaylyn WaddellDepartment of Pediatrics, University of Maryland School of Medicine, 655 W. Baltimore St., Baltimore, MD 21201, USA.
Tibor KristianDepartment of Anesthesiology and the Center for Shock, Trauma and Anesthesiology (S.T.A.R.), University of Maryland School of Medicine, 685 Baltimore Street, Baltimore, MD 21201, USA.ORCID 0000-0002-1804-8999

Funding

The inflammatory mechanisms underlying olfactory dysfunction in prognosis of TBI progression to dementiaR01AG077541 · NIA · UNIVERSITY OF MARYLAND BALTIMORE · PI Shaolin Liu, Junfang Wu · 2022 to 2026
$3.1M
Targeting mitochondrial Complex I in neonatal hypoxia-ischemiaR01NS122777 · NINDS · UNIVERSITY OF MARYLAND BALTIMORE · PI BRIAN M POLSTER, JAYLYN Waddell WADDELL · 2022 to 2026
$2.5M
Using NAD+ precursor for treatment of global cerebral ischemiaR01NS119275 · NINDS · UNIVERSITY OF MARYLAND BALTIMORE · PI KRISTIAN, TIBOR · 2021 to 2025
$1.9M
BLRD VA I01 BX004895NIA NIH HHS R01 AG077541NIH HHS 1R01AG077541-03A1NIH HHS 1R01NS119275-04A1NIH HHS 1R01NS122777-03A1NINDS NIH HHS R01 NS119275NINDS NIH HHS R01 NS122777Veterans Affairs BX004895
6 · The paper itself

Abstract

backgroundAcetylation of α-tubulin is an important post-translational modification that helps maintain microtubules' stability and dynamics, including axonal transport, cell signaling, and overall neuronal integrity. This study investigates sex-based differences in alcohol-induced acetylation of α-tubulin in mouse cerebellum.

methodsAdult, 3-month-old male and female C57BL/6 mice were administered 20% ethanol intraperitoneally. The cerebellum was dissected at 30 min, 1 h, 2 h, and 4 h post-injection. Expression levels of cerebellar acetylation of α-tubulin and enzymes mediating acetylation/deacetylation were analyzed by Western blot. The downstream product of ethanol metabolism, acetyl-CoA, was quantified by HPLC.

resultsIn males, α-tubulin acetylation levels increased significantly as early as 30 min post-ethanol injection, whereas females exhibited increased acetylation at a later time point, after 1 h. These sex-specific changes coincided with alterations in acetyl-CoA levels that increased significantly at 15 min in males and 1 h in females following ethanol administration. Furthermore, the level of acetyltransferase that acetylates tubulin increased significantly at 30 min in males and 1 h in females. Notably, however, no significant changes were observed in the level of the tubulin deacetylating enzyme, HDAC6, in either sex.

conclusionsOur data demonstrate that these sex differences stem from variations in expression levels of tubulin acetyltransferase (αTAT1), and the rate of ethanol metabolism-related acetyl-CoA production between male and female animals.

Indexed as

acetylationacetyl-CoAcerebellumethanolα-tubulin

Identifiers

PMID40309791
PMCPMC12025013

What Socratic holds

Textmetadata
LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.