Evidence map›Paper›PMID 40313723›Full record

ReviewFrontiers in plant science2025

Recent trends and advances in chloroplast engineering and transformation methods.

Muralikrishna Narra, Issei Nakazato, Brittany Polley, Shin-Ichi Arimura, Grant N Woronuk, Pankaj K Bhowmik

Abstract readReview
In one paragraph

Review in Frontiers in plant science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Review
  7. HarnessingFrontiers in plant science · 2026
    Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Muralikrishna NarraAquatic and Crop Resource Development, National Research Council of Canada (NRC), Saskatoon, SK, Canada.
Issei NakazatoGraduate School of Agriculture and Life Sciences, The University of Tokyo, Tokyo, Japan.
Brittany PolleyAquatic and Crop Resource Development, National Research Council of Canada (NRC), Saskatoon, SK, Canada.
Shin-Ichi ArimuraGraduate School of Agriculture and Life Sciences, The University of Tokyo, Tokyo, Japan.
Grant N WoronukRelica Genomics, Saskatoon, SK, Canada.
Pankaj K BhowmikAquatic and Crop Resource Development, National Research Council of Canada (NRC), Saskatoon, SK, Canada.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Chloroplast transformation technology has become a powerful platform for generating plants that express foreign proteins of pharmaceutical and agricultural importance at high levels. Chloroplasts are often chosen as attractive targets for the introduction of new agronomic traits because they have their own genome and protein synthesis machinery. Certain valuable traits have been genetically engineered into plastid genomes to improve crop yield, nutritional quality, resistance to abiotic and biotic stresses, and the production of industrial enzymes and therapeutic proteins. Synthetic biology approaches aim to play an important role in expressing multiple genes through plastid engineering, without the risk of pleiotropic effects in transplastomic plants. Despite many promising laboratory-level successes, no transplastomic crop has been commercialized to date. This technology is mostly confined to model species in academic laboratories and needs to be expanded to other agronomically important crop species to capitalize on its significant commercial potential. However, in recent years, some transplastomic lines are progressing in field trials, offering hope that they will pass regulatory approval and enter the marketplace. This review provides a comprehensive summary of new and emerging technologies employed for plastid transformation and discusses key synthetic biology elements that are necessary for the construction of modern transformation vectors. It also focuses on various novel insights and challenges to overcome in chloroplast transformation.

Indexed as

biolisticschloroplast transformationCRISPRmetabolic engineeringnanotechnologyRNAiSWNTssynthetic biology

Identifiers

PMID40313723
PMCPMC12043724

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.