Evidence mapPaperPMID 40320524Full record

ArticleStem cell research & therapy2025

Genome editing of TXNIP in human pluripotent stem cells for the generation of hepatocyte-like cells and insulin-producing islet-like aggregates.

Leonardo Traini, Javier Negueruela, Bernat Elvira, Wadsen St-Pierre-Wijckmans, Valerie Vandenbempt, Carlos E Buss, Ao Li, Israel Pérez-Chávez, Francisco Ribeiro-Costa, Mariana Nunes and 5 more

Abstract read
In one paragraph

Article in Stem cell research & therapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Leonardo Traini *Signal Transduction and Metabolism Laboratory, Université Libre de Bruxelles, Route de Lennik 808, B-1070, Brussels, Belgium.
Javier Negueruela *Signal Transduction and Metabolism Laboratory, Université Libre de Bruxelles, Route de Lennik 808, B-1070, Brussels, Belgium.
Bernat ElviraSignal Transduction and Metabolism Laboratory, Université Libre de Bruxelles, Route de Lennik 808, B-1070, Brussels, Belgium.
Wadsen St-Pierre-WijckmansSignal Transduction and Metabolism Laboratory, Université Libre de Bruxelles, Route de Lennik 808, B-1070, Brussels, Belgium.
Valerie VandenbemptSignal Transduction and Metabolism Laboratory, Université Libre de Bruxelles, Route de Lennik 808, B-1070, Brussels, Belgium.
Carlos E BussSignal Transduction and Metabolism Laboratory, Université Libre de Bruxelles, Route de Lennik 808, B-1070, Brussels, Belgium.
Ao LiSignal Transduction and Metabolism Laboratory, Université Libre de Bruxelles, Route de Lennik 808, B-1070, Brussels, Belgium.
Israel Pérez-ChávezSignal Transduction and Metabolism Laboratory, Université Libre de Bruxelles, Route de Lennik 808, B-1070, Brussels, Belgium.
Francisco Ribeiro-CostaSignal Transduction and Metabolism Laboratory, Université Libre de Bruxelles, Route de Lennik 808, B-1070, Brussels, Belgium.
Mariana NunesSignal Transduction and Metabolism Laboratory, Université Libre de Bruxelles, Route de Lennik 808, B-1070, Brussels, Belgium.
Joris MessensVIB-VUB Center for Structural Biology, Vlaams Instituut Voor Biotechnologie, B-1050, Brussels, Belgium.
Daria EzeriņaVIB-VUB Center for Structural Biology, Vlaams Instituut Voor Biotechnologie, B-1050, Brussels, Belgium.
David C HayInstitute for Regeneration and Repair, Centre for Regenerative Medicine, University of Edinburgh, Edinburgh, EH16 4UU, UK.
Mayank BansalSignal Transduction and Metabolism Laboratory, Université Libre de Bruxelles, Route de Lennik 808, B-1070, Brussels, Belgium. mayank.bansal@ulb.be.
Esteban N GurzovSignal Transduction and Metabolism Laboratory, Université Libre de Bruxelles, Route de Lennik 808, B-1070, Brussels, Belgium. esteban.gurzov@ulb.be.ORCID http://orcid.org/0000-0003-4642-0273

Funding

H2020 European Research Council GA817940Juvenile Diabetes Research Foundation United States of America 2-SRA-2024-1566-S-B
6 · The paper itself

Abstract

backgroundThioredoxin-interacting protein (TXNIP) plays a role in regulating endoplasmic reticulum (ER) and oxidative stress, which disrupt glucose homeostasis in diabetes. However, the impact of TXNIP deficiency on the differentiation and functionality of human stem cell-derived somatic metabolic cells remains unclear.

methodsWe used CRISPR-Cas12a genome editing to generate TXNIP-deficient (TXNIP

resultsTXNIP deficiency significantly increased H1-hESC proliferation without affecting pluripotency, viability, or differentiation potential into HLCs and SC-islets. Bulk RNA-sequencing of thapsigargin-treated TXNIP

conclusionsOur study demonstrates that TXNIP deficiency does not improve the differentiation or functionality of HLCs and SC-islets. We present the generation and characterisation of TXNIP

Indexed as

Carrier ProteinsGene EditingHepatocytesInsulin-Secreting CellsPluripotent Stem CellsThioredoxinsAnimalsCell DifferentiationFemaleHuman Embryonic Stem CellsHumansInsulinIslets of LangerhansMaleMiceMice, Inbred NODCarrier ProteinsInsulinThioredoxinsTXNIP protein, humanCRISPR-Cas12aHepatocyte-like cellsStem cell-derived isletsStem cellsTXNIP

Identifiers

PMID40320524
PMCPMC12051322

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.