Evidence map›Paper›PMID 40323508›Full record

ArticleNaunyn-Schmiedeberg's archives of pharmacology2025

Harmaline attenuates pain and inflammation: role of IL-1β, oxidative stress, nitric oxide and cyclo-oxygenase.

Satyam Chaubey, Lovedeep Singh

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Article in Naunyn-Schmiedeberg's archives of pharmacology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Satyam ChaubeyUniversity Institute of Pharma Sciences, Chandigarh University, Mohali, Punjab, 140413, India.
Lovedeep SinghUniversity Institute of Pharma Sciences, Chandigarh University, Mohali, Punjab, 140413, India. lovedeep992s@gmail.com.ORCID http://orcid.org/0000-0002-2903-4293

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Harmaline, a naturally occurring β-carboline alkaloid found in plants such as Peganum harmala and Banisteriopsis caapi, exhibits various pharmacological activities, including antioxidant and neuroprotective properties. Its free radical-scavenging ability contributes to cellular protection against oxidative stress. Based on these properties, the present study aimed to explore the antinociceptive and anti-inflammatory effects of harmaline using acetic acid-induced writhing and carrageenan-induced paw edema mice models. Mice were treated with acetic acid to induce writhing, and carrageenan was administered to induce paw edema. Following carrageenan administration, the animals were euthanized, and paw tissues were harvested for biochemical analysis. Harmaline significantly reduced the frequency of acetic acid-induced writhing and attenuated carrageenan-induced inflammation, as indicated by a decrease in paw thickness. Biochemical analysis revealed that harmaline (5 mg/kg) reduced lipid peroxidation and preserved GSH levels in paw tissues, highlighting its antioxidant effects. Furthermore, harmaline treatment lowered IL-1β levels and upregulated Nrf-2, indicating modulation of inflammatory and antioxidant signaling. Pre-treatment with substance P and L-arginine reversed harmaline's effects on writhing and paw edema, suggesting the involvement of cyclo-oxygenase and nitric oxide pathways. These pre-treatments also reversed the harmaline-induced reduction in TBARS and IL-1β levels, as well as elevation of GSH and Nrf-2 levels, further supporting the involvement of these pathways. Overall, this study demonstrates that harmaline effectively alleviates pain and inflammation through the inhibition of COX and nitric oxide pathways, alongside the activation of Nrf-2-antioxidant signaling. These findings support harmaline's potential as a therapeutic agent for pain and inflammation, warranting further research into its clinical applications.

Indexed as

AnalgesicsAnti-Inflammatory AgentsHarmalineInflammationPainAcetic AcidAnimalsAntioxidantsCarrageenanDisease Models, AnimalEdemaInterleukin-1betaMaleMiceNitric OxideOxidative StressAcetic AcidAnalgesicsAnti-Inflammatory AgentsAntioxidantsCarrageenanHarmalineIL1B protein, mouseInterleukin-1betaNitric OxideProstaglandin-Endoperoxide SynthasesCOX-inhibitionHarmalineInflammationNitric oxideNrf-2Oxidative stressPain

Identifiers

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.