Evidence map›Paper›PMID 40326149›Full record

ArticleVeterinary and comparative oncology2025

Impacts of Vincristine and Prednisolone Chemotherapy on the Canine Gut Microbiota in Dogs Undergoing Treatment for Lymphoma.

Juan Aragon, Annika M Weber, Madison Tipton, Jennifer Thomsen, Hend Ibrahim, Kristen Weishaar, Sangeeta Rao, Jan S Suchodolski, Jonathan Stockman, Elizabeth P Ryan and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary and comparative oncology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Juan AragonDepartment of Environmental and Radiological Health Sciences, College of Veterinary Medicine and Biomedical Sciences; Colorado State University, Fort Collins, Colorado, USA.
Annika M WeberDepartment of Food Science and Human Nutrition, Colorado State University, Fort Collins, Colorado, USA.
Madison TiptonDepartment of Environmental and Radiological Health Sciences, College of Veterinary Medicine and Biomedical Sciences; Colorado State University, Fort Collins, Colorado, USA.
Jennifer ThomsenDepartment of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences; Colorado State University, Fort Collins, Colorado, USA.
Hend IbrahimDepartment of Environmental and Radiological Health Sciences, College of Veterinary Medicine and Biomedical Sciences; Colorado State University, Fort Collins, Colorado, USA.
Kristen WeishaarDepartment of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Flint Animal Cancer Center, Colorado State University, Fort Collins, Colorado, USA.ORCID https://orcid.org/0000-0003-2827-2246
Sangeeta RaoDepartment of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences; Colorado State University, Fort Collins, Colorado, USA.
Jan S SuchodolskiDepartment of Small Animal Clinical Sciences, Gastrointestinal Laboratory, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, Texas, USA.
Jonathan StockmanDepartment of Environmental and Radiological Health Sciences, College of Veterinary Medicine and Biomedical Sciences; Colorado State University, Fort Collins, Colorado, USA.
Elizabeth P RyanDepartment of Environmental and Radiological Health Sciences, College of Veterinary Medicine and Biomedical Sciences; Colorado State University, Fort Collins, Colorado, USA.
Nora Jean NealonDepartment of Environmental and Radiological Health Sciences, College of Veterinary Medicine and Biomedical Sciences; Colorado State University, Fort Collins, Colorado, USA.

Funding

CSU Infectious Disease Research and Response Training ProgramT32AI162691 · NIAID · COLORADO STATE UNIVERSITY · PI DEAN, GREGG A · 2021 to 2025
$2.1M
Colorado State University: Department of Clinical Sciences- Center for Companion Animal Studies- Young Investigator Grant ProgramEldred FoundationNational Institute of Allergy and Infectious Diseases of the National Institutes of Health T32AI162691NIAID NIH HHS T32 AI162691United States Department of Agriculture-National Institute of Food and Agriculture Higher Education Multicultural Scholars Program
6 · The paper itself

Abstract

Chemotherapy can have adverse gastrointestinal effects in dogs and people. The objective of this study was to assess the impact of vincristine and prednisolone/prednisone, as part of a CHOP chemotherapy [cyclophosphamide, hydroxydaunorubicin, oncovin (vincristine) and prednisolone/prednisone] protocol, on gastrointestinal dysbiosis in dogs with lymphoma. We hypothesised the first week of chemotherapy (administration of vincristine and prednisolone/prednisone, VCR/Pred) produces compositional and functional shifts in the canine faecal microbiota that are associated with increased dysbiosis. Faecal samples from canine lymphoma patients (n = 25) were compared for microbiota and metabolites before (pre-chemotherapy) and after the first week of VCR/Pred (post-chemotherapy). A dysbiosis index (DI) was calculated for each dog via quantitative PCR of seven bacterial taxa established for altered ratios in canine gastrointestinal dysbiosis: Faecalibacterium, Turicibacter, Escherichia coli, Streptococcus, Blautia, Fusobacterium and Peptacetobacter hiranonis (formerly Clostridium hiranonis ). There was a significant increase in the DI post-chemotherapy compared to pre-chemotherapy (p = 0.021) concurrent with a significant decrease in faecal P. hiranonis concentrations post-chemotherapy (p = 0.0003). 16S rRNA amplicon sequencing analysis revealed a significant decrease in Enterococcaceae post-chemotherapy (p = 0.013). Targeted faecal lipid profiling identified markers of host and bacterial metabolic dysfunction that were altered following chemotherapy, including significant decreases in arachidonate (p = 0.0015), nervonate (p = 0.027), cholestanol (p = 0.011) and campesterol (p = 0.0035). These findings support that shifts in gut microbiota structure and function may contribute to gastroenteritis in dogs following the first week of VCR/Pred. Gut dysbiosis measures are important for improved treatment options that alleviate gastrointestinal complications associated with chemotherapy in animals and people.

Indexed as

Antineoplastic Combined Chemotherapy ProtocolsDog DiseasesGastrointestinal MicrobiomeLymphomaPrednisoloneVincristineAnimalsDogsDysbiosisFecesFemaleMalePrednisonePrednisolonePrednisoneVincristinebile acidcanine gut microbiomechemotherapydysbiosisfatty acidlymphoma

Identifiers

PMID40326149
PMCPMC12353578

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.