Evidence map›Paper›PMID 40326504›Full record

ArticleJournal of separation science2025

A HILIC-IM-MS-Based Pharmacometabodynamic Study of the Effects of Orally Administered Gefitinib on the Polar Urinary Metabolic Phenotypes of C57Bl6 Mice.

Adam King, Lee A Gethings, Robert S Plumb, Ian D Wilson

Abstract read
In one paragraph

Article in Journal of separation science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Adam KingWaters Corporation, Wilmslow, UK.
Lee A GethingsWaters Corporation, Wilmslow, UK.
Robert S PlumbWaters Corporation, Milford, Massachusetts, USA.
Ian D WilsonDivision of Systems Medicine, Department of Metabolism, Digestion and Reproduction, Imperial College, Burlington Danes Building, London, UK.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The effects of the anilinoquinazoline tyrosine kinase inhibitor (TKI) gefitinib on the polar urinary metabolome of mice following oral administration (50 mg/kg) were studied over the 24 h period post dose. Analysis was performed using a hydrophilic interaction liquid chromatographic separation (HILIC) hyphenated to cyclic ion mobility (cIM) and mass spectrometry (MS). This investigation revealed numerous time-dependent changes in the polar urinary metabolic phenotype of gefitinib-dosed mice that mirrored the plasma concentrations and urinary excretion of the drug and its metabolites. These changes showed both relative increases and decreases in the amounts of various endogenous metabolites, including 8-hydroxydeoxyguanosine, thymidine, acetylcarnitine, isobutyrylcarnitine, myristoylglycine, 3-phenylpropionylglycine, cyclic AMP, 19-oxotestosterone, and 3'-asparagine-AMP. These changes were generally seen to be greatest at the time of the highest plasma and urinary concentrations of gefitinib. The concordance of these effects on the urinary metabolome with plasma/urine drug concentrations strongly implies a range of pharmacometabodynamic effects pointing to mechanism-based regulation of a number of endogenous metabolic pathways by gefitinib.

Indexed as

QuinazolinesAdministration, OralAnimalsChromatography, LiquidGefitinibHydrophobic and Hydrophilic InteractionsMaleMass SpectrometryMetabolomeMiceMice, Inbred C57BLPhenotypeGefitinibQuinazolinesmetabolic pathwaysmetabolite identificationpolar metabolomeurinary metabotypes

Identifiers

PMID40326504
PMCPMC12053960

What Socratic holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.