Evidence map›Paper›PMID 40328936›Full record

ArticleCommunications medicine2025

Structural analysis of a micron-sized deposit of Cu

Keiichi Iwaya, Terumasa Nagase, Tamotsu Zako, Yoshiyuki Itoh, Takashima Yoshiko, Hisae Arai, Nobumasa Nagami, Takashi Ishikawa, Haruhiko Sugimura

Abstract read
In one paragraph

Article in Communications medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Keiichi IwayaDepartment of Pathology, Kyoundo Hospital, Sasaki Foundation, Chiyoda-ku, Tokyo, Japan. iwaya@po.kyoundo.jp.ORCID http://orcid.org/0000-0003-1839-1209
Terumasa NagaseNoritake Clinic, Ushiku, Ibaraki, Japan.
Tamotsu ZakoDepartment of Chemistry and Biology, Graduate School of Science and Engineering, Ehime University, Matsuyama, Ehime, Japan.ORCID http://orcid.org/0000-0001-7106-5766
Yoshiyuki ItohApplication Management Department, and Solution Development Center, JEOL Ltd., Akishima, Tokyo, Japan.ORCID http://orcid.org/0000-0003-1905-1783
Takashima YoshikoApplication Management Department, and Solution Development Center, JEOL Ltd., Akishima, Tokyo, Japan.ORCID http://orcid.org/0009-0009-0724-8408
Hisae AraiDepartment of Pathology, Kyoundo Hospital, Sasaki Foundation, Chiyoda-ku, Tokyo, Japan.
Nobumasa NagamiForensic Scientific Laboratory, Hyogo Prefectural Police H.Q., Chuo-Ku, Kobe, Japan.ORCID http://orcid.org/0000-0002-5976-2267
Takashi IshikawaDepartment of Breast Oncology, Tokyo Medical University, Shinjyuku-ku, Tokyo, Japan.
Haruhiko SugimuraSasaki Institute, Sasaki Foundation, Chiyoda-ku, Tokyo, Japan.

Funding

MEXT | Japan Society for the Promotion of Science (JSPS) 15K08597MEXT | Japan Society for the Promotion of Science (JSPS) 17K08708
6 · The paper itself

Abstract

backgroundProtein misfolding is a hallmark of aging, resulting in diabetes and neuroendocrine disorders. Insulin preparations also form aggregates known as insulin balls. Aggregated insulin preparations usually form amyloids and are stable in subcutaneous tissue, some specimens are cytotoxic to cultured cells.

methodsA multilayered structural analysis of the detailed morphology of 21 insulin balls was performed by connecting regions of interest along spatial axes. Gross and optical microscopic findings, Raman spectrometric analysis using formalin-fixed paraffin-embedded block specimens, matrix-assisted laser desorption/ionization-time of flight-mass spectrometry, microfocus X-ray computed tomography, scanning electron microscopy-energy dispersive X-ray spectroscopy analysis, and transmission electron microscopy analysis were performed.

resultsHere, we show the presence of 100 µm Cu

conclusionsThe largest amount of Cu

Identifiers

PMID40328936
PMCPMC12056176

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.