Evidence map›Paper›PMID 40332412›Full record

ArticleInternational journal of molecular sciences2025

miRNAs from Zebrafish Embryo Extracts Inhibit Breast Cancer Invasiveness and Migration by Modulating miR-218-5p/PI3K Pathway.

Noemi Monti, Daniele Antinori, Sara Proietti, Aurora Piombarolo, Alessandro Querqui, Guglielmo Lentini, Domenico Liguoro, Michele Aventaggiato, Marco Lucarelli, Andrea Pensotti and 4 more

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Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Noemi MontiDepartment of Experimental Medicine, Sapienza University of Rome, 00161 Rome, Italy.ORCID 0000-0002-7512-7984
Daniele AntinoriDepartment of Experimental Medicine, Sapienza University of Rome, 00161 Rome, Italy.
Sara ProiettiSystem Biology Group Lab, Sapienza University of Rome, 00161 Rome, Italy.
Aurora PiombaroloDepartment of Experimental Medicine, Sapienza University of Rome, 00161 Rome, Italy.
Alessandro QuerquiDepartment of Experimental Medicine, Sapienza University of Rome, 00161 Rome, Italy.
Guglielmo LentiniDepartment of Experimental Medicine, Sapienza University of Rome, 00161 Rome, Italy.ORCID 0009-0000-9773-4630
Domenico LiguoroSAFU Laboratory, Department of Research, Advanced Diagnostics and Technological Innovation, Translational Research Area, IRCCS Regina Elena National Cancer Institute, 00144 Rome, Italy.
Michele AventaggiatoDepartment of Experimental Medicine, Sapienza University of Rome, 00161 Rome, Italy.ORCID 0000-0002-9466-0047
Marco LucarelliDepartment of Experimental Medicine, Sapienza University of Rome, 00161 Rome, Italy.ORCID 0000-0002-5538-2494
Andrea PensottiResearch Unit of Philosophy of Science and Human Development, University Campus Bio-Medico of Rome, 00128 Rome, Italy.
Alessandro GiulianiEnvironment and Health Department, Istituto Superiore di Sanità, 00161 Rome, Italy.ORCID 0000-0002-4640-804X
Marco TafaniDepartment of Experimental Medicine, Sapienza University of Rome, 00161 Rome, Italy.ORCID 0000-0001-7128-810X
Andrea FusoDepartment of Experimental Medicine, Sapienza University of Rome, 00161 Rome, Italy.ORCID 0000-0001-9467-5671
Mariano BizzarriDepartment of Experimental Medicine, Sapienza University of Rome, 00161 Rome, Italy.ORCID 0000-0003-0408-4136

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Herein, we demonstrate that soluble factors extracted from the distinct phases of the development of zebrafish embryos (ZFEs) exhibit a specific miRNA profile. We removed proteins and concentrated miRNAs in different phase-related samples, which we investigated further. We observed that ZFEs modulate miRNA expression in both normal and cancerous breast cells, significantly inhibiting the invasiveness and motility of triple-negative breast cancer cells. Namely, ZFEs reactivate the synthesis of miR-218-5p in cancerous cells, leading to the downregulation of PI3K, which consequently alters the distribution of phosphoinositides (such as PIP2/PIP3). Moreover, the silencing of miR-218-5p abolished the ZFE effects. Restoring a proper PIP2/PIP3 ratio is crucial for promoting the regression of the malignant phenotype. Phenotypic reversion follows the extensive cytoskeleton rearrangement and the re-emergence of E-cadherin/β-catenin complexes. In addition, ZFEs antagonize the Epithelial Mesenchymal Transition (EMT) by modulating several pathways, including the TCTP-p53 axis. Overall, these results show that embryo extracts enriched with fish miRNAs reactivate endogenous miR-218-5p in cancerous cells, which in turn downregulates critical pathways involved in tumor progression and metastasis.

Indexed as

Breast NeoplasmsMicroRNAsPhosphatidylinositol 3-KinasesZebrafishAnimalsCell Line, TumorCell MovementEmbryo, NonmammalianEpithelial-Mesenchymal TransitionFemaleGene Expression Regulation, NeoplasticHumansNeoplasm InvasivenessSignal TransductionMicroRNAsMIRN218 microRNA, humanPhosphatidylinositol 3-KinasesE-cadherinembryo morphogenetic factorsepithelial–mesenchymal transitionmicroRNAsmiR-218-5pPI3Ktumor reversion

Identifiers

PMID40332412
PMCPMC12027887

What Socratic holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.