Evidence map›Paper›PMID 40333337›Full record

ArticleVaccines2025

Naive and Memory B Cell BCR Repertoires in Individuals Immunized with an Inactivated SARS-CoV-2 Vaccine.

Renato Kaylan Alves de Oliveira França, Pedro Henrique Aragão Barros, Jacyelle Medeiros Silva, Hitallo Guilherme Costa Fontinele, Andrea Queiroz Maranhão, Marcelo de Macedo Brigido

Abstract read
In one paragraph

Article in Vaccines, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Trial
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Renato Kaylan Alves de Oliveira FrançaDepartment of Cellular Biology, Institute of Biological Science, University of Brasília, Brasilia 70910-900, DF, Brazil.ORCID 0000-0003-4930-0971
Pedro Henrique Aragão BarrosDepartment of Cellular Biology, Institute of Biological Science, University of Brasília, Brasilia 70910-900, DF, Brazil.ORCID 0009-0001-6834-5756
Jacyelle Medeiros SilvaDepartment of Cellular Biology, Institute of Biological Science, University of Brasília, Brasilia 70910-900, DF, Brazil.
Hitallo Guilherme Costa FontineleDepartment of Cellular Biology, Institute of Biological Science, University of Brasília, Brasilia 70910-900, DF, Brazil.ORCID 0000-0002-4531-5781
Andrea Queiroz MaranhãoDepartment of Cellular Biology, Institute of Biological Science, University of Brasília, Brasilia 70910-900, DF, Brazil.ORCID 0000-0002-1946-7191
Marcelo de Macedo BrigidoDepartment of Cellular Biology, Institute of Biological Science, University of Brasília, Brasilia 70910-900, DF, Brazil.ORCID 0000-0002-7136-7059

Funding

Coordenação de Aperfeicoamento de Pessoal de Nível Superior 001Fundação de Apoio à Pesquisa do Distrito Federal 00193-00001263/2021-12Fundação Universidade de Brasília DPI-COPEI 7181
6 · The paper itself

Abstract

backgroundThe COVID-19 pandemic has spurred a global race for a preventive vaccine, with a few becoming available just one year after describing this novel coronavirus disease. Among these are inactivated virus vaccines like CoronaVac (Sinovac Biotech), which are used in several countries to reduce the pandemic's effects. However, its use was associated with low protection, particularly against novel virus variants that quickly appeared in the following months. Vaccines play a crucial role in activating the immune system to combat infections, with Memory B-cells being a key part of this mechanism, eliciting protective neutralizing antibodies. This work focused on studying B-cell memory repertoire after two consecutive doses of CoronaVac. METHODOLOGY: Memory B-cells were isolated from five CoronaVac vaccinated and five pre-pandemic individuals and subsequently stimulated in vitro before high-throughput Illumina sequencing of the Heavy Chain Variable repertoire.

resultsWe observed a shift in the VH repertoire with increased HCDR3 length and enrichment of IGVH 3-23, 3-30, 3-7, 3-72, and 3-74 for IgA BCRs and IGHV 4-39 and 4-59 for IgG BCRs. A high expansion of IgA-specific clonal populations was observed in vaccinated individuals relative to pre-pandemic controls, accompanied by shared IgA variable heavy chain (VH) sequences among memory B cells across different vaccine recipients of IgA clones was also observed in vaccinated individuals compared to pre-pandemic controls, with several IgA VH sharing between memory B cells from different vaccines. Moreover, a high convergence was observed among vaccinees and SARS-CoV-2 neutralizing antibody sequences found in the CoV-abDab database.

conclusionThese data show the ability of CoronaVac to elicit antibodies with characteristics similar to those previously identified as neutralizing antibodies, supporting its protective efficacy. Furthermore, this analysis of the immunological repertoire in the context of viral infections reinforces the importance of immunization in generating convergent antibodies for the antiviral response.

Indexed as

BCRCoronaVacmemory B cellSARS-CoV-2VH repertoire

Identifiers

PMID40333337
PMCPMC12031002

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.