Evidence map›Paper›PMID 40333970›Full record

ArticleScience advances2025

Hypermethylation of DNA impairs megakaryogenesis in delayed platelet recovery after allogeneic hematopoietic stem cell transplantation.

Yaqiong Tang, Xiaofei Song, Ziyan Zhang, Yifang Yao, Tingting Pan, Jiaqian Qi, Lijun Xia, Depei Wu, Yue Han

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Science advances, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. SteamedNutrients · 2025
    Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yaqiong TangNational Clinical Research Center for Hematologic Diseases, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou, China.ORCID 0000-0002-0382-1969
Xiaofei SongNational Clinical Research Center for Hematologic Diseases, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou, China.ORCID 0000-0002-7021-2434
Ziyan ZhangNational Clinical Research Center for Hematologic Diseases, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou, China.ORCID 0000-0002-2001-2354
Yifang YaoSuzhou Hongci Hematology Hospital, Suzhou, China.
Tingting PanNational Clinical Research Center for Hematologic Diseases, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou, China.
Jiaqian QiNational Clinical Research Center for Hematologic Diseases, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou, China.
Lijun XiaNational Clinical Research Center for Hematologic Diseases, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou, China.ORCID 0000-0002-9586-7971
Depei WuNational Clinical Research Center for Hematologic Diseases, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou, China.ORCID 0000-0002-6312-3863
Yue HanNational Clinical Research Center for Hematologic Diseases, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou, China.ORCID 0000-0002-7560-7195

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Delayed platelet recovery (DPR) is a common and complex complication of hematopoietic stem cell transplantation (HSCT) with unclear mechanisms and poor patient outcomes. Emerging evidence suggests that impaired megakaryogenesis plays a critical role in the development of DPR. In this study, we report remarkable hypermethylation within CG islands of HSCs and megakaryocyte progenitor cells (MKPs) in patients with DPR. Treatment with the hypomethylating agent decitabine reduced methylated CG levels in MKPs and enhanced megakaryocyte production in mice. In addition, we found that DNMT3A negatively regulated megakaryogenesis in megakaryocyte lineages derived from primary HSCs. Transplantation with HSC-overexpressed DNMT3A impeded platelet engraftment following HSCT in mice. We further demonstrated that DNMT3A was directly bound to and methylated ETS1 and RUNX1 during megakaryogenesis. These findings highlight abnormal DNA methylation in DPR and indicate that hypomethylating agents may improve megakaryocyte proliferation and differentiation by targeting DNMT3A-mediated methylation.

Indexed as

Blood PlateletsDNA MethylationHematopoietic Stem Cell TransplantationThrombopoiesisAnimalsCell DifferentiationCore Binding Factor Alpha 2 SubunitDecitabineDNA (Cytosine-5-)-MethyltransferasesDNA Methyltransferase 3AFemaleHematopoietic Stem CellsHumansMaleMegakaryocyte Progenitor CellsMegakaryocytesCore Binding Factor Alpha 2 SubunitDecitabineDNA (Cytosine-5-)-MethyltransferasesDNA Methyltransferase 3ADNMT3A protein, humanDnmt3a protein, mouse

Identifiers

PMID40333970
PMCPMC12057652

What Socratic holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.