Evidence map›Paper›PMID 40340802›Full record

ArticleBMC gastroenterology2025

M64HCl, a focal adhesion kinase activator, promotes intestinal mucosal healing in rats.

Guiming Liu, Ahmed Adham R Elsayed, Louis Boafo Kwantwi, Ricardo Gallardo-Macias, Vadim J Gurvich, Marc D Basson

Abstract read
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In one paragraph

Article in BMC gastroenterology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Guiming LiuDepartment of Biomedical Sciences, Northeast Ohio Medical University College of Medicine, 4209 State Route 44, Rootstown, OH, USA. gliu@neomed.edu.ORCID http://orcid.org/0000-0002-9292-6198
Ahmed Adham R ElsayedDepartment of Biomedical Sciences, Northeast Ohio Medical University College of Medicine, 4209 State Route 44, Rootstown, OH, USA.ORCID http://orcid.org/0000-0002-1227-4426
Louis Boafo KwantwiDepartment of Biomedical Sciences, Northeast Ohio Medical University College of Medicine, 4209 State Route 44, Rootstown, OH, USA.ORCID http://orcid.org/0000-0002-4015-2127
Ricardo Gallardo-MaciasInstitute for Therapeutics Discovery and Development, Department of Medicinal Chemistry, College of Pharmacy, University of Minnesota, Minneapolis, MN, USA.ORCID http://orcid.org/0009-0008-1486-8261
Vadim J GurvichInstitute for Therapeutics Discovery and Development, Department of Medicinal Chemistry, College of Pharmacy, University of Minnesota, Minneapolis, MN, USA.ORCID http://orcid.org/0000-0002-5420-1639
Marc D BassonDepartment of Biomedical Sciences, Northeast Ohio Medical University College of Medicine, 4209 State Route 44, Rootstown, OH, USA. mbasson@neomed.edu.ORCID http://orcid.org/0000-0001-9696-2789

Funding

Midwest Biomedical Accelerator Consortium: MBArCU01HL152410 · NHLBI · UNIVERSITY OF MISSOURI-COLUMBIA · PI FAY, WILLIAM P, GRANT, SHEILA ANN · 2019 to 2023
$5.6M
National Institutes of Health's National Center for Advancing Translational Sciences UL1TR002494NIH HHS U01HL152410
6 · The paper itself

Abstract

backgroundIntestinal mucosal injury may arise from various factors. While many drugs target the causative factors, none directly stimulate mucosal wound healing. We found that the specific focal adhesion kinase (FAK) activator, M64HCl, promotes intestinal mucosal healing in mice. This study aims to further validate the therapeutic impact of M64HCl on intestinal mucosal repair in rats as a second species.

methodsWistar rats were assigned to one of four groups: normal control, 1-day injury + vehicle, 4-day injury + vehicle, or 4-day injury + M64HCl. Intestinal injury was induced by serosally applying 75% acetic acid. Immediately after injury, rats received either a continuous infusion of M64HCl (25 mg/kg/day) or its vehicle (saline). Four days post-injury, blood was drawn to measure M64HCl levels and assess liver and kidney function. The intestines were removed and opened, ulcer areas were photographed for size quantification, and tissues were fixed for histological and immunohistochemical analysis.

resultsM64HCl substantially reduced ulcer area on gross examination, while histological analysis showed alleviation of pathological changes with M64HCl treatment. Immunohistochemical analysis confirmed increased immunoreactivity for phosphorylated FAK in the epithelium adjacent to the injury in M64HCl-treated rats. However, there was no change in the percentage of Ki67-positive cells in each crypt at the edge of the ulcer area. Serum creatinine, ALT, and AST levels did not differ between the 4-day injury groups with or without M64HCl treatment.

conclusionsM64HCl, a water-soluble FAK activator, promotes acetic acid-induced ulcer healing in rats and may be useful in treating gastrointestinal mucosal injury.

Indexed as

Focal Adhesion Protein-Tyrosine KinasesIntestinal MucosaWound HealingAcetic AcidAnimalsDisease Models, AnimalMaleRatsRats, WistarAcetic AcidFocal Adhesion Protein-Tyrosine KinasesFocal adhesion kinaseMucosal healingSmall intestine, mucosal injury

Identifiers

PMID40340802
PMCPMC12063223

What Socratic holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.