Evidence map›Paper›PMID 40346455›Full record

ArticleBMC genomics2025

Molecular regulation of whole genome DNA methylation in heat stress response of dairy cows.

Yuze Yang, Yumei Chen, Lirong Hu, Congcong Zhang, Gong Chen, Lingling Hou, Qing Xu, Yachun Wang, Min Li

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PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yuze YangCollege of Life Sciences and Bioengineering, Beijing Jiaotong University, Beijing, 100044, China.
Yumei ChenCollege of Life Sciences and Bioengineering, Beijing Jiaotong University, Beijing, 100044, China.
Lirong HuKey Laboratory of Animal Genetics, Breeding and Reproduction, MARA, National Engineering Laboratory for Animal Breeding, Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
Congcong ZhangCollege of Life Sciences and Bioengineering, Beijing Jiaotong University, Beijing, 100044, China.
Gong ChenCollege of Life Sciences and Bioengineering, Beijing Jiaotong University, Beijing, 100044, China.
Lingling HouCollege of Life Sciences and Bioengineering, Beijing Jiaotong University, Beijing, 100044, China.
Qing XuCollege of Life Sciences and Bioengineering, Beijing Jiaotong University, Beijing, 100044, China. qingxu@bjtu.edu.cn.
Yachun WangKey Laboratory of Animal Genetics, Breeding and Reproduction, MARA, National Engineering Laboratory for Animal Breeding, Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China. wangyachun@cau.edu.cn.
Min LiCollege of Life Science and Technology, Inner Mongolia Normal University, Huhhot, 010022, China. limin_8123@126.com.

Funding

Earmarked Fund for CARS-36 CARS-36Program for Changjiang Scholar and Innovation Research Team in University IRT_15R62Qinghai Science and Technology Major Program 2021-NK-A5
6 · The paper itself

Abstract

backgroundHeat stress seriously affects the production and health of dairy cows and is a key factor limiting the sustainable development of the dairy industry. DNA methylation serves as an important epigenetic regulatory mechanism closely associated with an animal's response to heat stress. However, the specific molecular mechanism of DNA methylation in cows' heat stress response is not fully understood.

resultsIn this study, whole genome bisulfite sequencing analysis of blood identified 49861 specific differentially methylated regions corresponding to 7613 differentially methylated genes between spring and summer dairy cows. Among them, 4069 the promoter region of differentially methylated genes were significantly enriched in key biological pathways such as substance transport, reactive oxygen species metabolism, signal transduction, and energy metabolism. By integrating the expression data of 4069 promoter differentially methylated genes, 157 genes were further screened, and their DNA methylation levels were negatively correlated with gene expression. The changes in DNLZ, GNAS, and SMAD5 genes were most significant, and network analysis showed that DNLZ gene has high connectivity in the protein-protein interaction network, indicating its potential key function in heat stress response. Experimental verification shows that under heat stress conditions, the methylation level of CpG islands in the promoter region of DNLZ gene significantly increases, and its methylation level is significantly negatively correlated with gene expression level. The Dual-luciferase reporter assays using constructs containing the DNLZ promoter reporter gene experiment further confirms that promoter methylation significantly inhibits DNLZ transcriptional activity, and the higher the degree of methylation, the stronger the inhibitory effect.

conclusionsThe research results provide new insights into the mechanism of heat stress-related DNA methylation in dairy cows, clarify the key roles of genes such as DNLZ, and provide potential target genes and epigenetic markers for the cultivation of heat-resistant dairy cows.

Indexed as

DNA MethylationHeat-Shock ResponseAnimalsCattleCpG IslandsEpigenesis, GeneticFemaleGene Expression RegulationGenomePromoter Regions, GeneticWhole Genome SequencingDairy cowsDMGDNA methylationHeat stressWGBS

Identifiers

PMID40346455
PMCPMC12065190

What Socratic holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.