ArticleMarine biotechnology (New York, N.Y.)2025
Sex-Biased Gene Expression of RNAi Pathway Components in the Sea Lice Caligus rogercresseyi.
Article in Marine biotechnology (New York, N.Y.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Authors and funding
4 authors.
Funding
Abstract
RNA interference (RNAi) is a conserved mechanism for post-transcriptional gene regulation and a critical process of arthropod immunity. This study investigates RNAi-associated genes in Caligus rogercresseyi, an ectoparasitic sea louse that poses significant challenges to salmon aquaculture. In that regard, 16 RNAi-associated genes were identified by in silico analysis, including Cr-AGO1, Cr-CNOT1, Cr-DCR, Cr-SND1, and Cr-XRN1. Phylogenetic analysis demonstrated clustering with homologous sequences from other arthropods, particularly the ectoparasitic copepod Lepeophtheirus salmonis. RNA-Seq analyses revealed developmentally regulated expression patterns, with RNAi-associated genes clustered into four distinct expression profiles. Quantitative PCR (qPCR) validation confirmed significant male-biased expression for several key genes, including Cr-AGO1 (109-fold increase), Cr-DCR (22-fold), Cr-XRN1 (22-fold), Cr-SND1 (fourfold), and Cr-CNOT1 (threefold), suggesting potential roles in male reproductive processes such as spermatogenesis. Cr-DDX6, Cr-Drosha, and Cr-XPO5, potentially involved in oocyte development and RNA transport, exhibited female-biased expression. These results provide new insights into RNAi-associated gene expression in C. rogercresseyi, uncovering significant developmental and sex-biased expression patterns. Characterizing these critical genes establishes a foundation for exploring control strategies based on the RNAi process, targeting sex-biased and developmentally essential genes. Such treatments could reduce reproductive success in sea lice while minimizing environmental impact, offering a sustainable alternative for managing caligidosis in aquaculture.
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Registered trials
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