The effects of green light-emitting diode irradiation and inducer factors on the differentiation of human adipose tissue-derived mesenchymal cells into myocardial-like cells.

Elahe Mousanejad, Massood Ezzatabadipour, Masoumeh Baghalishahi, Mohammad Shojaei, Shayan Vafaei, Ali Afgar, Seyed Noureddin Nematollahi-Mahani

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Article in Lasers in medical science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Elahe MousanejadKerman physiology Research Center, Institute of Neuropharmacology and Department of Anatomy, Kerman University of Medical Sciences, Kerman, Iran.

Massood EzzatabadipourKerman physiology Research Center, Institute of Neuropharmacology and Department of Anatomy, Kerman University of Medical Sciences, Kerman, Iran.

Masoumeh BaghalishahiKerman physiology Research Center, Institute of Neuropharmacology and Department of Anatomy, Kerman University of Medical Sciences, Kerman, Iran.

Mohammad Shojaei

Shayan VafaeiMashhad University of Medical Sciences, Mashhad, Iran.

Ali AfgarKerman physiology Research Center, Institute of Neuropharmacology and Department of Anatomy, Kerman University of Medical Sciences, Kerman, Iran.

Seyed Noureddin Nematollahi-MahaniKerman physiology Research Center, Institute of Neuropharmacology and Department of Anatomy, Kerman University of Medical Sciences, Kerman, Iran. nnematollahi@kmu.ac.ir.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Physical factors like LED light can influence cell behavior, impacting stem cell proliferation and differentiation. Stem cell-based therapies offer a promising alternative to heart transplantation for cardiac conditions like myocardial infarction. This study evaluated the effects of green LED light (530 nm) and a biochemical inducer cocktail (oxytocin and ascorbic acid) on human adipose tissue-derived mesenchymal stem cells (hAD-MSCs) differentiation into cardiomyocyte-like cells. hAD-MSCs were treated with LED, the cocktail, and their combination. Cardiomyogenic markers NKX2.5, GATA4, cTnI, and CX43 were assessed via real-time PCR and western blotting. LED and the cocktail enhanced cardiac gene expression and protein synthesis, but their combination showed significantly higher effects, especially on day 7. This study demonstrates for the first time that green LED light combined with oxytocin and ascorbic acid can enhance hAD-MSC cardiac differentiation, supporting its potential in regenerative therapies following human studies.

Indexed as

Adipose TissueCell DifferentiationLasers, SemiconductorMesenchymal Stem CellsMyocytes, CardiacAscorbic AcidCells, CulturedConnexin 43GATA4 Transcription FactorGreen LightHomeobox Protein Nkx-2.5HumansTroponin IAscorbic AcidConnexin 43GATA4 protein, humanGATA4 Transcription FactorGJA1 protein, humanHomeobox Protein Nkx-2.5NKX2-5 protein, humanTroponin I

Identifiers

PMID40347405

DOI10.3389/fcell.2022.985298

What Socratic holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.