Evidence mapPaperPMID 40348318Full record

ArticleDevelopmental biology2025

Estrogen-related receptor is required in adult Drosophila females for germline stem cell maintenance.

Anna B Zike, Madison G Abel, Sophie A Fleck, Emily D DeWitt, Lesley N Weaver

Abstract read
In one paragraph

Article in Developmental biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Structural modeling supports an interaction between thebioRxiv : the preprint server for biology · 2026
    Article
  2. RNA Sequencing in AdultbioRxiv : the preprint server for biology · 2026
    Article
  3. Article
  4. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Anna B ZikeDepartment of Biology, Indiana University, Bloomington, IN, 47405, USA.
Madison G AbelDepartment of Biology, Indiana University, Bloomington, IN, 47405, USA.
Sophie A FleckDepartment of Biology, Indiana University, Bloomington, IN, 47405, USA.
Emily D DeWittDepartment of Biology, Indiana University, Bloomington, IN, 47405, USA.
Lesley N WeaverDepartment of Biology, Indiana University, Bloomington, IN, 47405, USA. Electronic address: lnweaver@iu.edu.

Funding

Regulation of tissue stem cell lineages by nuclear receptor signalingR35GM150517 · TRUSTEES OF INDIANA UNIVERSITY · 2025 to 2025
$379k
NIGMS NIH HHS R00 GM127605NIGMS NIH HHS R35 GM150517
6 · The paper itself

Abstract

Adult tissue function is dependent on intrinsic factors that mediate stem cell self-renewal and proliferation in response to changes in physiology and the environment. The estrogen-related receptor (ERR) subfamily of orphan nuclear receptors are major transcriptional regulators of metabolism and animal physiology. In mammals, ERRs (NR3B1, NR3B2, NR3B3) have roles in regulating mitochondrial biosynthesis, lipid metabolism, as well as stem cell maintenance. The sole Drosophila ERR ortholog promotes larval growth by establishing a metabolic state during the latter half of embryogenesis. In addition, ERR is required in adult Drosophila males to coordinate glycolytic metabolism with lipid synthesis and within the testis to regulate spermatogenesis gene expression and fertility. Despite extensive work characterizing the role of ERR in Drosophila metabolism, whether ERR has a conserved requirement in regulating stem cell behavior has been understudied. To determine whether ERR regulates stem cell activity in Drosophila, we used the established adult female germline stem cell (GSC) lineage as a model. We found that whole-body ERR knockout in adult females using conditional heat shock-driven FLP-FRT recombination significantly decreases GSC number and glycolytic enzyme expression in GSCs. In addition, we found that ERR activity is required cell-autonomously in the adult female germline for maintenance of GSCs; whereas ERR regulation of GSCs is independent of its activity in adult female adipocytes. Our results highlight an ancient and conserved role for ERRs in the regulation of stem cell self-renewal.

Indexed as

Drosophila melanogasterDrosophila ProteinsGerm CellsReceptors, EstrogenStem CellsAnimalsFemaleGlycolysisMaleDrosophila ProteinsERR protein, DrosophilaReceptors, EstrogenNR3B1NR3B2NR3B3Nuclear receptorOogenesis

Identifiers

PMID40348318
PMCPMC13143219

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.