Evidence map›Paper›PMID 40349795›Full record

ArticleBioresource technology2025

pH adjustment increases biofuel production from inhibitory switchgrass hydrolysates.

Lillian M Barten, Johnathan G Crandall, Dan Xie, Jose Serate, Evan Handowski, Annie Jen, Katherine A Overmyer, Joshua J Coon, Chris Todd Hittinger, Robert Landick and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioresource technology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. OxyR regulates the oxidative stress response inApplied and environmental microbiology · 2026
    Article
  2. Article
  3. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors.

Lillian M BartenDOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, WI 53706, USA.
Johnathan G CrandallDOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, WI 53706, USA; Wisconsin Energy Institute, Laboratory of Genetics, Center for Genomic Science Innovation, J. F. Crow Institute for the Study of Evolution, University of Wisconsin-Madison, Madison, WI 53706, USA.
Dan XieDOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, WI 53706, USA.
Jose SerateDOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, WI 53706, USA.
Evan HandowskiDOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, WI 53706, USA.
Annie JenDOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, WI 53706, USA; Department of Biomolecular Chemistry, University of Wisconsin-Madison, WI 53706, USA.
Katherine A OvermyerDOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, WI 53706, USA; Department of Biomolecular Chemistry, University of Wisconsin-Madison, WI 53706, USA; Morgridge Institute for Research, Madison, WI 53706, USA.
Joshua J CoonDOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, WI 53706, USA; Department of Biomolecular Chemistry, University of Wisconsin-Madison, WI 53706, USA; Department of Chemistry, University of Wisconsin-Madison, WI 53706, USA; Morgridge Institute for Research, Madison, WI 53706, USA.
Chris Todd HittingerDOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, WI 53706, USA; Wisconsin Energy Institute, Laboratory of Genetics, Center for Genomic Science Innovation, J. F. Crow Institute for the Study of Evolution, University of Wisconsin-Madison, Madison, WI 53706, USA.
Robert LandickDOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, WI 53706, USA; Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA; Department of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA.
Yaoping ZhangDOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, WI 53706, USA.
Trey K SatoDOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, WI 53706, USA. Electronic address: tksato@glbrc.wisc.edu.

Funding

PREDOCTORAL TRAINING PROGRAM IN GENETICST32GM007133 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI PERNA, NICOLE T · 1985 to 2023
$16.5M
NIGMS NIH HHS T32 GM007133
6 · The paper itself

Abstract

Biofuels derived from renewable and sustainable lignocellulosic biomass, such as switchgrass, offer a promising means to limit greenhouse gas emissions. However, switchgrass grown under drought conditions contains high levels of chemical compounds that inhibit microbial conversion to biofuels. Fermentation of drought switchgrass hydrolysates by engineered Saccharomyces cerevisiae and Zymomonas mobilis results in lower ethanol production than does fermentation of hydrolyzed switchgrass from a typical rainfall year. Here, it is demonstrated that this inhibitory effect can be alleviated by altering the pH of drought switchgrass hydrolysates produced by two different pretreatment methods: Ammonia Fiber Expansion (AFEX) and Soaking in Aqueous Ammonia (SAA). Fermentation rates and biofuel production by Saccharomyces cerevisiae and Zymomonas mobilis were higher at pH 5.8 than at pH 5.0 from all feedstock years and following both pretreatment methods. SAA pretreatment of drought switchgrass furthermore enabled increased fermentation rates and biofuel titers compared to AFEX pretreatment. A synthetic mimic of switchgrass hydrolysate was developed and identified relief from pH-dependent inhibition by lignocellulose-derived inhibitors as the cause of increased biofuel production above a pH of 5.0. These results demonstrate that SAA pretreatment and pH adjustment can significantly improve fermentation and biofuel production from inhibitory feedstocks by industrial microorganisms.

Indexed as

BiofuelsEthanolPanicumAmmoniaDroughtsFermentationGreenhouse GasesHydrogen-Ion ConcentrationLigninProtein HydrolysatesSaccharomyces cerevisiaeZymomonasAmmoniaBiofuelsEthanolGreenhouse GasesLigninlignocelluloseProtein Hydrolysates

Identifiers

PMID40349795
PMCPMC12129653

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.