ArticleAnimal reproduction2025
Egg-yolk- and a liposome-based extenders: refrigeration time and effects on ram semen quality.
Article in Animal reproduction, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed.
- Evaluation of milk-based and egg-based semen extenders for extended storage of ram semen at 4°C.Translational animal science · 2026Article
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Authors and funding
7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Sperm cells require time to adjust to lower temperatures. While some studies have established stabilization periods ranging from two to four hours for ovine semen, the exploration of longer periods of stabilization remains limited. The objective of the study is to evaluate the 12-hour refrigeration curve during ovine semen cryopreservation and the impact of a diluent medium containing egg yolk and liposomes. Eight mixed-breed rams (½ Dorper and ½ Santa Inês) provided two ejaculates each, divided into two groups. Group A used a commercial egg yolk-based diluent (Botu-Bov® - Botupharma Ltda, Brazil), while Group B used a commercial liposome-based diluent (OptiXcell®, IMV Technologies, France). Semen was packaged in French straws, cooled, cryopreserved, and thawed for analysis. Group A exhibited superior values (P < 0.05) in progressive motility (MP), progressive linear velocity (VSL), straightness (STR), and linearity (LIN) post-thawing and after 3 hours at 37°C (TTR). Conversely, Group B showed higher (P < 0.05) values for path velocity (VAP), curvilinear velocity (VCL), lateral head displacement amplitude (ALH) post-thawing, and VAP, VSL, VCL, and ALH after TTR. Flow cytometry revealed Group A's higher (P > 0.05) plasma and acrosomal membrane integrity and membrane stability. However, Group B exhibited greater (P > 0.05) superoxide anion generation and lipid peroxidation, indicative of higher oxidative stress. In conclusion, the egg yolk-based diluent outperformed the diluent containing liposomes in sperm kinetic parameters evaluated by CASA, although liposomes showed increased oxidative stress, 12 hours of refrigeration at 5.0°C is an alternative viable for semen cryopreservation in sheep.
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