Evidence map›Paper›PMID 40363839›Full record

ArticleMolecules (Basel, Switzerland)2025

Development and Characterization of pFluor50, a Fluorogenic-Based Kinetic Assay System for High-Throughput Inhibition Screening and Characterization of Time-Dependent Inhibition and Inhibition Type for Six Human CYPs.

Pratik Shriwas, Andre Revnew, Sarah Roo, Alex Bender, Kevin Miller, Christopher M Hadad, Thomas R Lane, Sean Ekins, Craig A McElroy

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecules (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Pratik ShriwasDivision of Medical Chemistry and Pharmacognosy, College of Pharmacy, The Ohio State University, Columbus, OH 43210, USA.
Andre RevnewDivision of Medical Chemistry and Pharmacognosy, College of Pharmacy, The Ohio State University, Columbus, OH 43210, USA.
Sarah RooDivision of Medical Chemistry and Pharmacognosy, College of Pharmacy, The Ohio State University, Columbus, OH 43210, USA.
Alex BenderDivision of Medical Chemistry and Pharmacognosy, College of Pharmacy, The Ohio State University, Columbus, OH 43210, USA.
Kevin MillerDepartment of Chemistry and Biochemistry, College of Arts and Sciences, The Ohio State University, Columbus, OH 43210, USA.ORCID 0000-0001-7749-6794
Christopher M HadadDepartment of Chemistry and Biochemistry, College of Arts and Sciences, The Ohio State University, Columbus, OH 43210, USA.ORCID 0000-0003-1211-4315
Thomas R LaneCollaborations Pharmaceuticals, Raleigh, NC 27606, USA.ORCID 0000-0001-9240-4763
Sean EkinsCollaborations Pharmaceuticals, Raleigh, NC 27606, USA.
Craig A McElroyDivision of Medical Chemistry and Pharmacognosy, College of Pharmacy, The Ohio State University, Columbus, OH 43210, USA.ORCID 0000-0001-5950-0120

Funding

Defense Threat Reduction Agency HDTRA11910020
6 · The paper itself

Abstract

Cytochrome P450s (CYPs) play an integral role in drug and xenobiotic metabolism in humans, and thus, understanding CYP inhibition and/or activation by new therapeutic candidates is an important step in the drug development process. Ideally, CYP inhibition/activation assays should be high-throughput, use commercially available components, allow for analysis of metabolism by the majority of human CYPs, and allow for kinetic analysis of inhibition type and time-dependent inhibition. Here, we developed pFluor50, a 384-well microtiter plate-based fluorogenic kinetic enzyme assay system using substrates metabolized by six human CYPs to generate fluorescent products and determined the Michaelis-Menten kinetics constants (K

Indexed as

Cytochrome P-450 Enzyme InhibitorsCytochrome P-450 Enzyme SystemFluorescent DyesHigh-Throughput Screening AssaysHumansKineticsCytochrome P-450 Enzyme InhibitorsCytochrome P-450 Enzyme SystemFluorescent DyesCYP450fluorescent probeshigh throughput screeningkinetic assaymetabolismplate reader

Identifiers

PMID40363839
PMCPMC12074421

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.