Evidence map›Paper›PMID 40365797›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025

AlphaFold-Guided Bespoke Gene Editing Enhances Field-Grown Soybean Oil Contents.

Jie Wang, Li Zhang, Shoudong Wang, Xin Wang, Suning Li, Pingping Gong, Mengyan Bai, Arnav Paul, Nathan Tvedt, Hengrui Ren and 13 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed.

  1. Article
  2. Review
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  5. Plant structural biology: Emerging technologies and future biological insights.The Plant journal : for cell and molecular biology · 2026
    Review
  6. Review
  7. Article
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  11. Review
  12. Article
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  16. AlphaFold-Guided Bespoke Gene Editing Enhances Field-Grown Soybean Oil Contents.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Article
  17. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

23 authors.

Jie WangCollege of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Li ZhangGuangdong Provincial Key Laboratory of Plant Adaptation and Molecular Design, Innovative Center of Molecular Genetics and Evolution, School of Life Sciences, Guangzhou University, Guangzhou, 510006, China.
Shoudong WangKey Laboratory of Soybean Molecular Design Breeding, State Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, 130102, China.
Xin WangGuangdong Provincial Key Laboratory of Plant Adaptation and Molecular Design, Innovative Center of Molecular Genetics and Evolution, School of Life Sciences, Guangzhou University, Guangzhou, 510006, China.
Suning LiJiangxi Province Key Laboratory of Oil Crops Genetic Improvement, Crop Institute, Jiangxi Academy of Agricultural Sciences, Nanchang, 330200, China.
Pingping GongFujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Haixia Institute of Science and Technology and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Mengyan BaiGuangdong Provincial Key Laboratory of Plant Adaptation and Molecular Design, Innovative Center of Molecular Genetics and Evolution, School of Life Sciences, Guangzhou University, Guangzhou, 510006, China.
Arnav PaulDepartment of Chemistry, University of Illinois Urbana-Champaign, Urbana, IL, 61801, USA.
Nathan TvedtCenter for Biophysics and Quantitative Biology, University of Illinois Urbana-Champaign, Urbana, IL, 61801, USA.
Hengrui RenGuangdong Provincial Key Laboratory of Plant Adaptation and Molecular Design, Innovative Center of Molecular Genetics and Evolution, School of Life Sciences, Guangzhou University, Guangzhou, 510006, China.
Maoxiang YangCollege of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Zhihui ZhangCollege of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Shaodong ZhouCollege of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Jiayi SunKey Laboratory of Soybean Molecular Design Breeding, State Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, 130102, China.
Xianjin WuCollege of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Huaqin KuangGuangdong Provincial Key Laboratory of Plant Adaptation and Molecular Design, Innovative Center of Molecular Genetics and Evolution, School of Life Sciences, Guangzhou University, Guangzhou, 510006, China.
Zhenghua DuFujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Haixia Institute of Science and Technology and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Yonghui DongFujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Haixia Institute of Science and Technology and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Xiaolei ShiHebei Laboratory of Crop Genetics and Breeding, Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang, 050035, China.
Meina LiGuangdong Provincial Key Laboratory of Plant Adaptation and Molecular Design, Innovative Center of Molecular Genetics and Evolution, School of Life Sciences, Guangzhou University, Guangzhou, 510006, China.
Diwakar ShuklaDepartment of Chemistry, University of Illinois Urbana-Champaign, Urbana, IL, 61801, USA.
Long YanHebei Laboratory of Crop Genetics and Breeding, Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang, 050035, China.
Yuefeng GuanGuangdong Provincial Key Laboratory of Plant Adaptation and Molecular Design, Innovative Center of Molecular Genetics and Evolution, School of Life Sciences, Guangzhou University, Guangzhou, 510006, China.ORCID https://orcid.org/0000-0002-1436-3828

Funding

China Agriculture Research System of MOF and MARA CARS-04-PS06National Key Research and Development Program of China 2023YFF1000203National Natural Science Foundation of China 32001504Natural Science Foundation of Hebei Province C2024301125Youth Innovation Promotion Association of Chinese Academy of Sciences 2023239
6 · The paper itself

Abstract

Enhancing the oil or protein content of soybean, a major crop for oil and protein production is highly desirable. GmSWEET10a encodes a sugar transporter that is strongly selected during domestication and breeding, increasing seed size and oil content. GmSWEET10b is functionally similar to GmSWEET10a, yet has not been artificially selected. Here, AlphaFold is used to find that C-terminal variants of GmSWEET10a can endow enhanced or reduced transport activity. Guided by AlphaFold, the functionality is improved for GmSWEET10a in terms of oil content through gene editing. Furthermore, novel GmSWEET10b haplotypes possessing strengthened or weakened sugar-transport capabilities that are absent in nature are engineered. Consequently, soybean oil content or protein content in independent GmSWEET10b gene-edited lines during multi-year and multi-site field trials is consistently increased, without negatively affecting yield. The study demonstrates that the combination of AlphaFold-guided protein design and gene editing has the potential to generate novel beneficial alleles, which can optimize protein function in the context of crop breeding.

Indexed as

Gene EditingGlycine maxSoybean OilPlant BreedingPlant ProteinsPlants, Genetically ModifiedPlant ProteinsSoybean OilAlphaFoldCRISPR–Cas9gene editingoil contentprecision breedingsoybeanSWEET transporter

Identifiers

PMID40365797
PMCPMC12199397

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.