Evidence map›Paper›PMID 40374167›Full record

ArticleJournal of proteome research2025

Comparative Proteomic Analysis Reveals Altered Ciliary Proteins in Sickle Cell Disease.

Ashraf M Mohieldin, Madison Spencer, Carter Bernal, Wala B Fadol, Ankan Gupta, Karthikeyan Thirugnanam, Claire Delahunty, Francisco Nunez, Amy Y Pan, Amanda M Brandow and 6 more

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of proteome research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Ashraf M MohieldinCollege of Graduate Studies, Master Program of Pharmaceutical Science, California Northstate University, Elk Grove, California 95757, United States.ORCID 0000-0001-8200-8898
Madison SpencerCollege of Graduate Studies, Master Program of Pharmaceutical Science, California Northstate University, Elk Grove, California 95757, United States.
Carter BernalCollege of Graduate Studies, Master Program of Pharmaceutical Science, California Northstate University, Elk Grove, California 95757, United States.
Wala B FadolDepartment of Clinical Science, College of Medicine, California Northstate University, Elk Grove, California 95757, United States.
Ankan GuptaDepartment of Pediatrics, Developmental Vascular Biology Program, Division of Neonatology, Children's Research Institute (CRI), Medical College of Wisconsin, Milwaukee, Wisconsin 53226, United States.
Karthikeyan ThirugnanamDepartment of Pediatrics, Developmental Vascular Biology Program, Division of Neonatology, Children's Research Institute (CRI), Medical College of Wisconsin, Milwaukee, Wisconsin 53226, United States.
Claire DelahuntyDepartment of Molecular Medicine, The Scripps Research Institute, La Jolla, California 92037, United States.
Francisco NunezDepartment of Pharmaceutical Sciences, School of Pharmacy, Chapman University, Irvine, California 92618, United States.
Amy Y PanDepartment of Pediatrics, Division of Bioinformatics and Quantitative Child Health, CRI, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, United States.
Amanda M BrandowDepartment of Pediatrics, Division of Hematology/Oncology/Bone Marrow Transplantation, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, United States.
Sean P PalecekDepartment of Chemical and Biological Engineering, College of Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States.ORCID 0000-0003-4917-5584
Kevin R RarickDepartment of Pediatrics, Division of Critical Care, CRI, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, United States.
Ramani RamchandranDepartment of Pediatrics, Developmental Vascular Biology Program, Division of Neonatology, Children's Research Institute (CRI), Medical College of Wisconsin, Milwaukee, Wisconsin 53226, United States.ORCID 0000-0002-3555-6119
Rahima ZennadiDepartment of Physiology, College of Medicine, The University of Tennessee Health Science, Memphis, Tennessee 38163, United States.
John YatesDepartment of Molecular Medicine, The Scripps Research Institute, La Jolla, California 92037, United States.ORCID 0000-0001-5267-1672
Surya M NauliDepartment of Pharmaceutical Sciences, School of Pharmacy, Chapman University, Irvine, California 92618, United States.ORCID 0000-0002-9676-0287

Funding

The roles of neuronal primary cilia in Alzheimer's diseaseR01HL147311 · NHLBI · CHAPMAN UNIVERSITY · PI ALACHKAR, AMAL, NAULI, SURYA · 2020 to 2023
$2.1M
Cilia as a biomarker of CNS vascular healthR33HL154254 · NHLBI · MEDICAL COLLEGE OF WISCONSIN · PI NAULI, SURYA, PALECEK, SEAN P · 2022 to 2024
$1.9M
NHLBI NIH HHS R01 HL147311NHLBI NIH HHS R33 HL154254
6 · The paper itself

Abstract

Sickle cell disease (SCD) is an inherited hemoglobinopathy characterized by sickle-shaped red blood cells (RBCs). Primary cilia are mechanosensory organelles and are projected in the lumen of blood vessels to detect blood flow. We previously reported that interaction between microvasculature endothelial cells and sickled RBCs resulted in altered blood flow that can elevate reactive oxygen species, leading to increased deciliation in SCD patients. However, the impact of deciliation mediated by sickled RBCs in the context of the ciliary protein profiles remains unclear. Here, we investigated cell-cilia stability under different physiological shear-stress magnitudes and examined cilia protein profiles in SCD, utilizing mouse models and human participants. Our results demonstrate that subjecting endothelial cilia to sickled RBCs at 5.0 dyn/cm

Indexed as

Anemia, Sickle CellCiliaProteomicsAnimalsDisease Models, AnimalEndothelial CellsErythrocytesFemaleHumansMaleMiceProtein Processing, Post-TranslationalReceptors, TransferrinReceptors, Transferrindiagnostic biomarkerextracellular vesiclesprimary ciliaproteomicsred blood cellssickle cell diseasesickle cell traitvascular damage

Identifiers

PMID40374167
PMCPMC12151070

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.