ArticleEnvironmental toxicology2025
MiR-1 Is Regulated by Hydrogen Peroxide via MAPK and Limits Cell Migration and Invasion.
Article in Environmental toxicology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed.
- MiR-1 Is Regulated by Hydrogen Peroxide via MAPK and Limits Cell Migration and Invasion.Environmental toxicology · 2025Article
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3 authors.
Funding
Abstract
MicroRNA-1 (miR-1) is a conserved microRNA that is highly expressed in skeletal and cardiac muscle tissues. Moreover, miR-1 regulates genes and has critical roles in cell migration and invasion. Downregulation of miR-1 has been found in many pathologies of numerous organs, including the lungs. What exactly contributes to the downregulation of miR-1 is not fully understood, and in the present study, we investigated whether ROS regulate miR-1 and its role in cell migration and invasion. A549 cells were grown and maintained in DMEM:F12 (1:1) and supplemented with 10% FBS and 1000 U of Penicillin/Streptomycin and maintained as recommended by the manufacturer (ATCC). Cell migration and invasion, IHC, Western blot, qPCR, ROS, miR-1 transfection, and qPCR were used to determine miR-1 regulation and its role in cell migration. Exogenous miR-1 decreased the formation of ROS and inhibited cell migration and invasion, whereas inhibition of miR-1 increased ROS formation and stimulated cell migration and invasion. Inhibition of miR-1 induced the formation of actin filaments contractile structures, whereas exogenous miR-1 limited the formation of these structures. Hydrogen peroxide significantly decreased miR-1 level, whereas inhibition of Nox4 had no effect on miR-1 level. Alpha amanitin did not decrease miR-1 level, whereas inhibition of NF-кB temporally decreased miR-1 level. This study demonstrates that ROS suppress miR-1 and that miR-1 is posttranscriptionally regulated via MAPK. Endogenous Nox4-dependent ROS are not involved in miR-1 regulation, whereas exogenous ROS regulates miR-1. NF-κB plays a key role in miR-1 regulation in both redox and nonredox environments. Moreover, Mir-1 limits cell migration and invasion even in the presence of ROS. TSP-1 is a major regulator of TGFβ and its expression is upregulated by ROS. Our work indicates ROS is a major regulator of miR-1 and TSP-1 and could be a potential therapeutic target to limit ROS- and non-ROS-mediated processes in lung cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.