ArticleJournal of advanced research2026
Overexpression of soybean flavonoid 3'-hydroxylase enhances plant salt tolerance by promoting ascorbic acid biosynthesis.
Article in Journal of advanced research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
7 citing papers in PubMed.
- Insights Into the Regulation of Salt Tolerance by Flavonoid and Cyanoamino Acid Metabolism in Sorghum-Sudangrass Hybrids.Plant direct · 2026Article
- Analysis of differential mechanisms in flavonoid synthesis in the exocarp of virescens and nigrescens fruits and development of KASP molecular markers.BMC plant biology · 2026Article
- ThePlants (Basel, Switzerland) · 2026Article
- Transcriptome Analysis Unveils the Crucial Role of Mitochondrial Oxidative Phosphorylation Pathways inPlants (Basel, Switzerland) · 2026Article
- Flavonoids in Plant Salt Stress Responses: Biosynthesis, Regulation, Functions, and Signaling Networks.Plants (Basel, Switzerland) · 2026Review
- Integrated physiological and multi-omics analyses reveal genotype-specific GABA responses associated with salt tolerance inFrontiers in plant science · 2026Article
- Root-centered sodium sequestration and transcriptomic regulation under salt and alkali stress in wild soybean (Glycine soja).Frontiers in plant science · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionSalt stress is a major cause of crop loss. Soybean (Glycine max), a globally vital legume crop, faces mounting yield constraints due to soil salinization. It is known that the flavonoid biosynthesis pathway involving flavonoid 3'-hydroxylase (F3'H) plays an important role in salt tolerance. However, the precise molecular basis of F3'H-mediated salt tolerance remains inadequately characterized.
objectivesThis study aimed to elucidate the function and explore the pleiotropic molecular basis of F3'H protein in soybean salt tolerance. Innovation on elite new crop varieties facilitates breeding and production applications on salt tolerance.
methodsWe employed CRISPR/Cas9-mediated knockout and Agrobacterium-based overexpression to generate GmF3'H allelic variants and ectopic expression in soybeans. Sanger sequencing and quantitative reverse transcription polymerase chain reaction (qRT-PCR) were used to confirm the specificity of gene editing and quantify expression levels in overexpression transgenic plants, respectively. As well as Subcellular localization analysis, Yeast two-hybrid (Y2H) assay, LUC activity assay and plant physiological measurements were carried out to elucidate the F3'H-mediated salt tolerance molecular basis in plants.
resultsIn this study, we identified the flavonoid 3' hydroxylase gene (GmF3'H) in soybeans, which as a master regulator of salt stress adaptation during seed germination and seedling stages in both soybean and Arabidopsis thaliana. Furthermore, our study revealed that the evolutionarily conserved F3'H protein competitively binds to photomorphogenic factor COP9 signalosome subunit 5B (CSN5B) and disrupts its interaction with GDP-mannose pyrophosphorylase 1 (VTC1), a key enzyme in ascorbate biosynthesis. This competitive inhibition redirects metabolic flux toward the L-galactose pathway, leading to an increase in ascorbic acid (AsA) biosynthesis. The enhanced AsA production subsequently improves seedling salt stress tolerance in plants by maintaining redox homeostasis through ROS scavenging.
conclusionThe discovery and characterization of F3'H-mediated salt tolerance provide a crucial framework for the genetic improvement of crops. This work provides new insights into plant salt stress tolerance and develops innovative strategies to enhance broad-spectrum salt tolerance, a crucial aspect for ensuring food security in crops.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.