Evidence map›Paper›PMID 40388447›Full record

ArticlePloS one2025

Vibrio harveyi plasmids as drivers of virulence in barramundi (Lates calcarifer).

Roisin Sullivan, Joy A Becker, Ruth N Zadoks, Carola Venturini, Ana I S Esteves, Suresh Benedict, Dani L Fornarino, Hannah Andrews, God'spower R Okoh, Vidya Bhardwaj and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Roisin SullivanSydney School of Veterinary Science, Faculty of Science, The University of Sydney, Camden, New South Wales, Australia.ORCID https://orcid.org/0009-0000-4535-8119
Joy A BeckerSchool of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Camden, New South Wales, Australia.
Ruth N ZadoksSydney School of Veterinary Science, Faculty of Science, The University of Sydney, Camden, New South Wales, Australia.
Carola VenturiniSydney School of Veterinary Science, Faculty of Science, The University of Sydney, Camden, New South Wales, Australia.
Ana I S EstevesElizabeth Macarthur Agricultural Institute, New South Wales Department of Primary Industries and Environment, Menangle, New South Wales, Australia.
Suresh BenedictBerrimah Veterinary Laboratory, Department of Agriculture and Fisheries, Northern Territory Government, Berrimah, Northern Territory, Australia.
Dani L FornarinoBerrimah Veterinary Laboratory, Department of Agriculture and Fisheries, Northern Territory Government, Berrimah, Northern Territory, Australia.
Hannah AndrewsBerrimah Veterinary Laboratory, Department of Agriculture and Fisheries, Northern Territory Government, Berrimah, Northern Territory, Australia.ORCID https://orcid.org/0009-0001-3385-6214
God'spower R OkohBerrimah Veterinary Laboratory, Department of Agriculture and Fisheries, Northern Territory Government, Berrimah, Northern Territory, Australia.
Vidya BhardwajBerrimah Veterinary Laboratory, Department of Agriculture and Fisheries, Northern Territory Government, Berrimah, Northern Territory, Australia.
Mark SistromBerrimah Veterinary Laboratory, Department of Agriculture and Fisheries, Northern Territory Government, Berrimah, Northern Territory, Australia.
Mark E WestmanSydney School of Veterinary Science, Faculty of Science, The University of Sydney, Camden, New South Wales, Australia.ORCID https://orcid.org/0000-0001-7400-1960
Nguyen Ngoc PhuocFaculty of Fisheries, University of Agriculture and Forestry, Hue University, Hue City, Vietnam.
Francisca SamsingSydney School of Veterinary Science, Faculty of Science, The University of Sydney, Camden, New South Wales, Australia.ORCID https://orcid.org/0000-0002-6343-2295

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Vibrio species are an emerging public and animal health risk in marine environments and the opportunistic bacterial pathogen Vibrio harveyi is a major disease risk for tropical aquaculture. Current understanding of virulence in V. harveyi is limited by strain-specific variability and complex host-pathogen dynamics. This study sought to integrate genomic investigation, phenotypic characterisation and in vivo challenge trials in barramundi (Lates calcarifer) to increase our understanding of V. harveyi virulence. We identified two hypervirulent isolates, Vh-14 and Vh-15 that caused 100% mortality in fish within 48 hours, and that were phenotypically and genotypically distinct from other V. harveyi isolates. Virulent isolates contained multiple plasmids, including a 105,412 bp conjugative plasmid with type III secretion system genes originally identified in Yersinia pestis. The emergence of this hypervirulent plasmid-mediated patho-variant poses a potential threat to the sustainable production of marine finfish in Southeast Asia, the Mediterranean and Australia. In addition, we observed an effect of temperature on phenotypic indicators of virulence with an increase in activity at 28°C and 34°C compared to 22°C. This suggests that temperature fluctuations associated with climate change may act as a stressor on bacteria, increasing virulence gene secretion and host adaptation. Our results utilising a myriad of technologies and tools, highlights the importance of a holistic view to virulence characterisation.

Indexed as

Fish DiseasesPerciformesPlasmidsVibrioVibrio InfectionsAnimalsVirulence

Identifiers

PMID40388447
PMCPMC12088062

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.