Evidence map›Paper›PMID 40389583›Full record

ArticlePsychopharmacology2025

Early maternal separation potentiates the impact of later social isolation in inducing depressive-like behavior via oxidative stress in adult rats.

Yating Chen, Jingjing Du, Mengzhu Lei, Na Ji, Wei Zhang, Chuanyu Li, Bo Zhang

Abstract read
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In one paragraph

Article in Psychopharmacology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yating Chen *Guangxi Key Laboratory of Brain and Cognitive Neuroscience, Faculty of Basic Medical Sciences, Guilin Medical University, #1 Zhiyuan Road, Lingui District, Guilin Guangxi, 541199, China.
Jingjing Du *Guangxi Key Laboratory of Brain and Cognitive Neuroscience, Faculty of Basic Medical Sciences, Guilin Medical University, #1 Zhiyuan Road, Lingui District, Guilin Guangxi, 541199, China.
Mengzhu LeiGuangxi Key Laboratory of Brain and Cognitive Neuroscience, Faculty of Basic Medical Sciences, Guilin Medical University, #1 Zhiyuan Road, Lingui District, Guilin Guangxi, 541199, China.
Na JiGuangxi Key Laboratory of Brain and Cognitive Neuroscience, Faculty of Basic Medical Sciences, Guilin Medical University, #1 Zhiyuan Road, Lingui District, Guilin Guangxi, 541199, China.
Wei ZhangGuangxi Key Laboratory of Brain and Cognitive Neuroscience, Faculty of Basic Medical Sciences, Guilin Medical University, #1 Zhiyuan Road, Lingui District, Guilin Guangxi, 541199, China.
Chuanyu LiGuangxi Key Laboratory of Brain and Cognitive Neuroscience, Faculty of Basic Medical Sciences, Guilin Medical University, #1 Zhiyuan Road, Lingui District, Guilin Guangxi, 541199, China. chuanyuli4@foxmail.com.
Bo ZhangGuangxi Key Laboratory of Brain and Cognitive Neuroscience, Faculty of Basic Medical Sciences, Guilin Medical University, #1 Zhiyuan Road, Lingui District, Guilin Guangxi, 541199, China. bozhangpp@foxmail.com.ORCID http://orcid.org/0000-0003-4518-6745

Funding

Guangxi Key Laboratory of Brain and Cognitive Neuroscience GKLBCN-20190101Guangxi science and technology base and talent special project GuiKe-AD20238023National Natural Science Foundation of China 32260200Natural Science Foundation of Guangxi Province 2025GXNSFAA069126, 2020GXNSFAA297134
6 · The paper itself

Abstract

rationaleIndividuals who have experienced early life stress (ELS) are more vulnerable to later life stress induced depression, which might attribute to ELS potentiated impact of later life stress. The presumption and neurobiological mechanisms involved require further validation and elucidation.

objectivesTo investigate impact of pre-weaning maternal separation (MS) on post-weaning social isolation (SI) in inducing depressive-like behavior, and involvement of central oxidative stress, glutamatergic and brain-derived neurotrophic factor (BDNF)/tyrosine kinase receptor B (TrkB) signaling in the process.

methodsMale offspring were exposed to MS, SI or maternal separation and social isolation (MSSI) stress, respectively. Subjects were treated with saline, antioxidant diallyl disulfide (DADS) (30 mg/kg, i.g.) or antidepressant fluoxetine (10 mg/kg, i.p.), for two weeks before behavioral tests in adolescents or adults. Depressive-like behavior was assessed with sucrose preference, forced swim and tail suspension tests. Concentrations of 4-hydroxynonenal (4-HNE), glutathione and superoxide dismutase in hippocampus and serum, and hippocampal protein expressions of glutamate transporter 1 (GLT-1), BDNF and TrkB were assessed by western blotting analysis.

resultsMSSI, rather than MS or SI, induced significant depressive-like behavior, in adults but not adolescents. Consistently, only MSSI significantly elevated 4-HNE, whereas inhibited GLT-1, BDNF and TrkB in adult hippocampus. MSSI induced behavioral and biochemical abnormalities in adults were reversed by DADS or fluoxetine treatment.

conclusionsEarly MS age-dependently potentiates later SI impact in inducing depressive-like behavior in male rats, through elevating oxidative stress and interrupting glutamatergic and BDNF/TrkB signaling in the brain. Results further suggest antioxidant treatment as a promising anti-depressant avenue.

Indexed as

DepressionMaternal DeprivationOxidative StressSocial IsolationStress, PsychologicalAnimalsAntidepressive AgentsAntioxidantsBehavior, AnimalBrain-Derived Neurotrophic FactorDisease Models, AnimalDisulfidesExcitatory Amino Acid Transporter 2FemaleFluoxetineHippocampusAntidepressive AgentsAntioxidantsBdnf protein, ratBrain-Derived Neurotrophic FactorDisulfidesExcitatory Amino Acid Transporter 2FluoxetineNtrk2 protein, ratReceptor, trkBAntioxidantBDNF/TrkBChronic stressDepressionGlutamateOxidative stress

Identifiers

What Socratic holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.