Evidence map›Paper›PMID 40389884›Full record

ArticleBMC ophthalmology2025

Unveiling the levels and significance of different serpin family proteins in aqueous humor dynamics.

Eliza Williams, Jeremy Altman, Saleh Ahmed, Garrett Jones, Anshuman Khadanga, Danny Alevy, Kathryn Bollinger, Amy Estes, Steven Safille, Tae Jin Lee and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC ophthalmology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Eliza WilliamsCenter for Biotechnology and Genomic Medicine, Medical College of Georgia, Augusta University, 1120 15th Street, CA4143, Augusta, GA, 30912, USA.
Jeremy AltmanCenter for Biotechnology and Genomic Medicine, Medical College of Georgia, Augusta University, 1120 15th Street, CA4143, Augusta, GA, 30912, USA.
Saleh AhmedCenter for Biotechnology and Genomic Medicine, Medical College of Georgia, Augusta University, 1120 15th Street, CA4143, Augusta, GA, 30912, USA.
Garrett JonesCenter for Biotechnology and Genomic Medicine, Medical College of Georgia, Augusta University, 1120 15th Street, CA4143, Augusta, GA, 30912, USA.
Anshuman KhadangaCenter for Biotechnology and Genomic Medicine, Medical College of Georgia, Augusta University, 1120 15th Street, CA4143, Augusta, GA, 30912, USA.
Danny AlevyCenter for Biotechnology and Genomic Medicine, Medical College of Georgia, Augusta University, 1120 15th Street, CA4143, Augusta, GA, 30912, USA.
Kathryn BollingerDepartment of Ophthalmology, Medical College of Georgia, Augusta University, Augusta, GA, 30912, USA.
Amy EstesDepartment of Ophthalmology, Medical College of Georgia, Augusta University, Augusta, GA, 30912, USA.
Steven SafilleCenter for Biotechnology and Genomic Medicine, Medical College of Georgia, Augusta University, 1120 15th Street, CA4143, Augusta, GA, 30912, USA.
Tae Jin LeeCenter for Biotechnology and Genomic Medicine, Medical College of Georgia, Augusta University, 1120 15th Street, CA4143, Augusta, GA, 30912, USA.
Shruti SharmaCenter for Biotechnology and Genomic Medicine, Medical College of Georgia, Augusta University, 1120 15th Street, CA4143, Augusta, GA, 30912, USA.
Ashok SharmaCenter for Biotechnology and Genomic Medicine, Medical College of Georgia, Augusta University, 1120 15th Street, CA4143, Augusta, GA, 30912, USA. assharma@augusta.edu.

Funding

Proteomic Biomarkers for Glaucomatous Optic NeuropathyR01EY029728 · NEI · AUGUSTA UNIVERSITY · PI SHARMA, ASHOK · 2019 to 2022
$1.5M
NEI NIH HHS R01 EY029728
6 · The paper itself

Abstract

backgroundAlterations in the constituents of the aqueous humor (AH) are associated with various ocular pathologies, including primary open-angle glaucoma (POAG). AH contains a variety of immunomodulatory molecules, including serine protease inhibitors (serpins), which regulate several proteolytic cascades such as coagulation, angiogenesis, and inflammation. The purpose of this study was to examine the levels of different serpins in human AH and their association with POAG pathology.

methodsThe abundance of all 37 serpins was determined using LC-MS/MS analysis in 289 human AH samples (cataract: n = 209; POAG: n = 80). The potential involvement of these serpins in POAG was examined by correlating their levels with clinical parameters such as intraocular pressure (IOP) and optic nerve damage.

resultsAmong the 37 serpins present in the human proteome, 26 were detected in aqueous humor. The thirteen most abundant serpins in AH include SERPINA1, SERPINF1, SERPINC1, SERPINA3, SERPING1, AGT, SERPINF2, SERPINA4, SERPINA6, SERPIND1, SERPINI1, SERPINA7, and SERPINA5. Seven serpins were downregulated in subjects with POAG, including SERPINI1 (FC = 0.26), SERPINA4 (FC = 0.40), SERPINA6 (FC = 0.42), SERPINA7 (FC = 0.46), SERPINC1 (FC = 0.74), AGT (FC = 0.76), and SERPING1 (FC = 0.78).

conclusionThis study highlights significant alterations in serpin levels within the AH of individuals with POAG. Sex-specific and race-specific differences in the levels of several serpins were also observed. Further studies are needed to clarify the specific mechanisms by which these serpins may contribute to POAG progression and to investigate their potential clinical relevance.

Indexed as

Aqueous HumorGlaucoma, Open-AngleSerpinsAgedAged, 80 and overBiomarkersChromatography, LiquidFemaleHumansIntraocular PressureMaleMiddle AgedTandem Mass SpectrometryBiomarkersSerpinsAqueous humorGlaucomaInflammationMass spectrometrySerpins

Identifiers

PMID40389884
PMCPMC12090511

What Socratic holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.