Evidence mapPaperPMID 40394458Full record

ArticleBMC genomics2025

Chromatin accessibility and transcriptomic profiles of sheep pituitary function associated with fecundity.

Shanglai Li, Bingru Zhao, Peiyong Chen, Yu Cai, Hui Xu, Chenbo Yan, Feng Wang, Yanli Zhang

Abstract read
In one paragraph

Article in BMC genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Shanglai Li *Hu Sheep Academy, Nanjing Agricultural University, Nanjing, 210095, China.
Bingru Zhao *Hu Sheep Academy, Nanjing Agricultural University, Nanjing, 210095, China.
Peiyong ChenHu Sheep Academy, Nanjing Agricultural University, Nanjing, 210095, China.
Yu CaiHu Sheep Academy, Nanjing Agricultural University, Nanjing, 210095, China.
Hui XuHu Sheep Academy, Nanjing Agricultural University, Nanjing, 210095, China.
Chenbo YanHu Sheep Academy, Nanjing Agricultural University, Nanjing, 210095, China.
Feng WangHu Sheep Academy, Nanjing Agricultural University, Nanjing, 210095, China.
Yanli ZhangHu Sheep Academy, Nanjing Agricultural University, Nanjing, 210095, China. zhangyanli@njau.edu.cn.

Funding

National key research and development program 2021YFF1000702Project of Seed Industry Revitalization in Jiangsu Province JBGS [2021]113
6 · The paper itself

Abstract

backgroundThe pituitary gland, a central regulator of the hypothalamic-pituitary-gonadal (HPG) axis, plays a pivotal role in reproductive efficiency by precisely controlling the secretion of gonadotropins, including follicle-stimulating hormone (FSH) and luteinizing hormone (LH). Chromatin accessibility enables physical interactions between promoters and chromatin-binding factors to drive the gene expression. Despite this mechanistic insight, the chromatin accessibility landscape of the sheep pituitary and its functional implications for reproductive traits remain largely unexplored. To address this knowledge gap, we performed an integrated multi-omics analysis of ATAC-seq and RNA-seq profiling of pituitary from sheep with divergent fecundity phenotypes.

resultsWe identified 1,567 differential accessibility regions (DARs) and 768 differentially expressed genes (DEGs). Functional enrichment analysis revealed that the DEGs were significantly associated with key signaling pathways, including neuroactive ligand-receptor interactions, the cAMP signaling pathway, and the calcium signaling pathway, suggesting their critical roles in pituitary-regulated reproductive functions. Based on integrative analysis of ATAC-seq and RNA-seq, we revealed several potentially key genes involved in gonadotropin secretion, such as CMKLR1, TAFA1, and PPP1R17. Furthermore, we identified novel transcription factors (TFs), including NR4A2 and MEF2, which may influence pituitary hormone secretion by modulating chromatin accessibility and gene expression.

conclusionsThis study systematically delineated the gene expression and chromatin accessibility of the pituitary and identified some key regulatory genes associated with gonadotropin secretion in sheep. Our integrated multi-omics analysis identifies critical molecular markers that may contribute to the genetic improvement of reproductive efficiency in ovine species.

Indexed as

ChromatinFertilityGene Expression ProfilingPituitary GlandTranscriptomeAnimalsFemaleSheepTranscription FactorsChromatinTranscription FactorsChromatin accessibilityFertilityGene expressionPituitary

Identifiers

PMID40394458
PMCPMC12090423

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.