Evidence map›Paper›PMID 40400169›Full record

ArticleThe Journal of infectious diseases2025

Seroprevalence of Dengue, Zika, and Chikungunya Viruses in Humans and Colocated Macaques in Thailand and Cambodia.

Nanthanida Mongkol, Sophana Chea, Somnang Man, Piseth Ly, Cheata Hou, Sokna Ly, Ratanak Sath, Chanthap Lon, Kry Hok, Huch Chea and 16 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of infectious diseases, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Observational
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

26 authors.

Nanthanida MongkolDepartment of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.ORCID 0000-0002-1719-6236
Sophana CheaInternational Center of Excellence in Research Cambodia, National Institute of Allergy and Infectious Diseases, Phnom Penh, Cambodia.ORCID 0000-0003-3187-3691
Somnang ManInternational Center of Excellence in Research Cambodia, National Institute of Allergy and Infectious Diseases, Phnom Penh, Cambodia.ORCID 0000-0002-2145-0954
Piseth LyInternational Center of Excellence in Research Cambodia, National Institute of Allergy and Infectious Diseases, Phnom Penh, Cambodia.ORCID 0009-0009-5212-2636
Cheata HouInternational Center of Excellence in Research Cambodia, National Institute of Allergy and Infectious Diseases, Phnom Penh, Cambodia.
Sokna LyInternational Center of Excellence in Research Cambodia, National Institute of Allergy and Infectious Diseases, Phnom Penh, Cambodia.
Ratanak SathInternational Center of Excellence in Research Cambodia, National Institute of Allergy and Infectious Diseases, Phnom Penh, Cambodia.
Chanthap LonInternational Center of Excellence in Research Cambodia, National Institute of Allergy and Infectious Diseases, Phnom Penh, Cambodia.ORCID 0000-0003-4901-5003
Kry HokKampong Speu Provincial Health Department, Ministry of Health, Kampong Speu, Cambodia.
Huch CheaNational Center for Parasitology, Entomology, and Malaria Control, Ministry of Health, Phnom Penh, Cambodia.ORCID 0000-0003-4791-9412
Rithea LeangNational Center for Parasitology, Entomology, and Malaria Control, Ministry of Health, Phnom Penh, Cambodia.ORCID 0000-0003-2645-7618
Rekol HuyNational Center for Parasitology, Entomology, and Malaria Control, Ministry of Health, Phnom Penh, Cambodia.ORCID 0000-0001-8552-3369
Sophia B LinCritical Care Medicine Department, National Institutes of Health Clinical Center, Bethesda, Maryland, USA.ORCID 0009-0000-4366-4681
Fanny Sae WangDepartment of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Daraka TongthainanFaculty of Veterinary Medicine, Rajamangala University of Technology Tawan-ok, Chonburi, Thailand.ORCID 0000-0003-1223-1335
Wirasak FungfuangDepartment of Zoology, Faculty of Science, Kasetsart University, Bangkok, Thailand.
Sudarat LijitipoomLopburi Provincial Public Health Office, Ministry of Public Health, Lopburi, Thailand.
Sarocha SuthisawatDepartment of Immunology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Nathamon KosoltanapiwatDepartment of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.ORCID 0000-0001-7465-0696
Natanon PanpethBungchawak Non-Hunting Area and Bungchawak Wildlife Management Development Center, Department of National Parks, Wildlife, and Plant Conservation, Suphanburi, Thailand.
Stephen S WhiteheadLaboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, USA.ORCID 0000-0001-6707-482X
Fabiano OliveiraInternational Center of Excellence in Research Cambodia, National Institute of Allergy and Infectious Diseases, Phnom Penh, Cambodia.ORCID 0000-0002-7924-8038
Rebecca C ChristoffersonDepartment of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana, USA.ORCID 0000-0003-2806-1131
Jessica E ManningInternational Center of Excellence in Research Cambodia, National Institute of Allergy and Infectious Diseases, Phnom Penh, Cambodia.
Kobporn BoonnakDepartment of Immunology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand.ORCID 0000-0002-7536-8964
Christina YekInternational Center of Excellence in Research Cambodia, National Institute of Allergy and Infectious Diseases, Phnom Penh, Cambodia.ORCID 0000-0001-8293-3901

Funding

Asia Joint Research Program P-18-52763Civilian Research and Development Foundation DAA3-19-64908Division of Intramural Research, National Institute of Allergy and Infectious Diseases
6 · The paper itself

Abstract

backgroundArboviruses-short for "arthropod-borne viruses"-are transmitted to humans and animals by infected arthropods. Aedes mosquito-borne arboviruses such as dengue virus (DENV), Zika virus (ZIKV), and chikungunya virus (CHIKV) impose major public health burdens in Southeast Asia. The potential role of sylvatic reservoirs, such as macaques, in maintaining arboviral transmission remains unclear.

methodsWe assessed the role of sylvatic reservoirs in arboviral circulation by examining serological evidence of exposure to DENV, ZIKV, and CHIKV among humans and macaques living in close proximity in endemic regions. Cross-sectional serosurveys were carried out during 2019-2022, involving 649 humans and 398 macaques colocated across 4 sites in Thailand (Hua Hin, Laem Chabang, and Muang Lop Buri) and Cambodia (Chbar Mon). Seropositivity was evaluated using plaque reduction neutralization tests (PRNT50).

resultsWe found overall higher seropositivity rates across arboviruses among human populations compared to macaques (38.5%-74.4% vs 0%-8.0%, respectively, using PRNT50 cutoff). Virus seroprevalence differed between Thai and Cambodian cohorts and age was the only significant predictor of human seropositivity in multivariate analyses.

conclusionsThe low seropositivity among macaques suggests a limited role of macaques in sustaining and amplifying urban arboviral cycles; rather, low-level macaque seropositivity may signal virus spillback from human populations.

Indexed as

Chikungunya FeverDengueZika Virus InfectionAdolescentAdultAgedAnimalsAntibodies, ViralCambodiaChikungunya virusChildChild, PreschoolCross-Sectional StudiesDengue VirusFemaleHumansAntibodies, ViralChikungunyadengueseroprevalenceZika

Identifiers

PMID40400169
PMCPMC12455315

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.