ArticleJournal of assisted reproduction and genetics2025
GDNF enhances HGF-induced tubulogenesis and organization of Sertoli cell.
Article in Journal of assisted reproduction and genetics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Tuning the Testicular Microenvironment for Enhancing Human Sertoli Cells Maturation and Functionality In Vitro.Advanced healthcare materials · 2026Article
- Sertoli cells-only syndrome: current clinical approaches and ongoing research trends.Frontiers in endocrinology · 2025Review
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Funding
Abstract
purposeHepatocyte growth factor (HGF), secreted by Sertoli cells, activates the c-Met receptor, facilitating seminiferous tubule formation. Glial cell-derived neurotrophic factor (GDNF) promotes embryonic Sertoli cell proliferation and cord formation. This study aimed to investigate whether exogenous GDNF contributes to HGF-induced in vitro organization of Sertoli cells in a Matrigel-based three-dimensional (3D) culture system.
methodsFour experimental groups were established using the 15P-1 Sertoli cell line: control, HGF-treated, GDNF-treated, and combined HGF- and GDNF-treated. Tubular structure length and branching were quantified using image analysis software, while the expression of ZO-1 protein was assessed via immunofluorescence staining in 3D Matrigel-based culture for 5 days. Additionally, Gfrα-1, Ret, Ncam, and Met mRNA expression in Sertoli cells were analyzed. The expression levels of ZO-1, c-MET, and p-c-MET were analyzed in two-dimensional (2D) culture after 5 days.
resultsQuantitative analysis revealed a significant increase in the length and branching of tubular-like structures when GDNF was co-administered with HGF, compared to other groups. Additionally, there was a notable increase in ZO-1 protein expression with the combined administration of GDNF and HGF, surpassing levels observed in the control group. Furthermore, co-administration of HGF and GDNF significantly elevated phospho-c-Met levels compared to the control group.
conclusionOur study reveals that exogenous GDNF enhances the HGF-induced tubular organization of Sertoli cells in vitro. The concurrent administration of GDNF and HGF markedly augments the formation of tubular structures and the expression of the ZO-1 protein, indicating a potential synergistic influence on the organizational and signaling cascades within Sertoli cells.
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