Evidence map›Paper›PMID 40410685›Full record

ArticleBMC plant biology2025

Comparative physiological and cytological analysis, and omics approach provide clues to the coloring mechanism of the pumpkin yellow stems.

Liting Deng, Jianning Luo, Haibin Wu, Xiaoxi Liu, Gangjun Zhao, Hao Gong, Xiaoming Zheng, Chaoqun Ni, Xueting Wang, Junxing Li

Abstract readComparative Study
In one paragraph

Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Liting DengGuangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Jinying Road No.66, Guangdong, 510640, Guangzhou, China.
Jianning LuoGuangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Jinying Road No.66, Guangdong, 510640, Guangzhou, China.
Haibin WuGuangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Jinying Road No.66, Guangdong, 510640, Guangzhou, China.
Xiaoxi LiuGuangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Jinying Road No.66, Guangdong, 510640, Guangzhou, China.
Gangjun ZhaoGuangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Jinying Road No.66, Guangdong, 510640, Guangzhou, China.
Hao GongGuangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Jinying Road No.66, Guangdong, 510640, Guangzhou, China.
Xiaoming ZhengGuangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Jinying Road No.66, Guangdong, 510640, Guangzhou, China.
Chaoqun NiGuangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Jinying Road No.66, Guangdong, 510640, Guangzhou, China.
Xueting WangGuangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Jinying Road No.66, Guangdong, 510640, Guangzhou, China.
Junxing LiGuangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Jinying Road No.66, Guangdong, 510640, Guangzhou, China. lijunxing@gdaas.cn.

Funding

the Guangdong Province Modern Vegetable Industry Technology System Project grant number 2024CXTD08the Guangzhou Science and Technology Planning Project grant number 2023A04J0821the National Bulk Vegetable Industry Technology System Guangzhou Comprehensive Experimental Station grant number CARS-23-G50the Open Research Fund of Guangdong Key Laboratory for New Technology Research on Vegetables grant number 2021KF-01the Science and Technology Innovation Strategy (Agricultural Research Main Force Construction) Project grant number R2023PY-JX006the Seed Industry Revitalization Project of Special Fund for Rural Revitalization Strategy of Gunagdong Province grant number 2024-440400-103010206-0002the Special Fund for Scientific Innovation Strategy-Construction of High Level Academy of Agriculture Science grant number R2022YJ-YB1001
6 · The paper itself

Abstract

backgroundThe abnormal chloroplast and pigment accumulation could lead to plant yellowing. However, the plant tissue chlorisis is species-specific and could display various phenotypes due to the genetic and environmental impacts. The molecular mechanisms underlying the plant stem yellowing are less understood than the flower or leaf coloring mechanisms. Herein, the physiological, cytological, transcriptome analysis, along with genome-wide association study (GWAS) were integrated to illustrate the processes relevant to pumpkin stem coloring.

resultsSimilar yet different variations were discovered in the pumpkin yellow stems. Low content of photosynthetic pigments, and impaired chloroplast thylakoid membrane were identified in the pumpkin yellowing stems, together with the presence of plastoglobules and starch grains. Elevated expression of genes in catabolism of chlorophylls and carotenoids was found in yellow stems, which may result in the failed accumulation of pigments and pumpkin stem chlorisis. Concurrently, increased expression of genes in chloroplast development, antioxidant protection, photosynthesis, and ribosome were found, which may act as compensation mechanisms for chloroplast defects. The integrated analysis of transcriptome and GWAS identified the up-regulated proteases and decreased kinesins in yellow stems, which could result in the breakdown of thylakoid systems, and the disability of photosynthetic pigments accumulation. Additionally, transcription factors could be involved in the regulation of the specific color change in pumpkin stems.

conclusionsThese findings provide clues into the molecular mechanisms of stem yellowing, and will facilitate the exploration of candidate targets as markers or genetic improvement through molecular breeding.

Indexed as

CucurbitaPigmentationPlant StemsChlorophyllChloroplastsGene Expression ProfilingGene Expression Regulation, PlantGenome-Wide Association StudyPhotosynthesisTranscriptomeChlorophyllCarotenoidChlorisisChlorophyllChloroplastPhotosynthesisPumpkin

Identifiers

PMID40410685
PMCPMC12100919

What Socratic holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.