Evidence map›Paper›PMID 40413658›Full record

ArticleCellular and molecular neurobiology2025

Screening, Analysis, and Validation of Endoplasmic Reticulum Stress-Related DEGs in Epilepsy.

Sunyena Shi, Xu Feng, Zhan Cao, Lin Wang, Mingjian Sun, Ziyi Zhao, Wei Sun

Abstract read
In one paragraph

Article in Cellular and molecular neurobiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Sunyena Shi *Department of Neurology, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150086, China.ORCID http://orcid.org/0009-0001-3379-6389
Xu Feng *Department of Neurology, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150086, China.ORCID http://orcid.org/0009-0005-4058-0868
Zhan Cao *Department of Neurology, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150086, China.ORCID http://orcid.org/0009-0006-3385-5429
Lin WangDepartment of Neurology, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150086, China.ORCID http://orcid.org/0009-0005-6002-9416
Mingjian SunDepartment of Neurology, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150086, China.ORCID http://orcid.org/0009-0007-6834-6912
Ziyi ZhaoDepartment of Neurology, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150086, China.ORCID http://orcid.org/0009-0008-2734-3528
Wei SunDepartment of Neurology, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150086, China. sunwei@hrbmu.edu.cn.ORCID http://orcid.org/0000-0002-0234-8954

Funding

the Second Affiliated Hospital of Harbin Medical University Innovative Scientific Research Fund for young and middle-aged people KYCX2019-19
6 · The paper itself

Abstract

To investigate the relationship between Endoplasmic Reticulum Stress (ERS) and epilepsy, as well as their biological functions. We downloaded the GSE143272 dataset from the GEO database, identified differentially expressed genes (DEGs), and cross-analyzed them with ERS-related genes from GeneCards and the Molecular Signatures Database (MSigDB). Protein-protein interaction (PPI) networks were constructed, and Hub genes were screened. ROC curve analysis was conducted to assess the diagnostic utility of these genes, followed by qRT-PCR validation. This study identified a total of 83 ERS-related DEGs in epilepsy. PPI network analysis revealed eight feature genes: C-X-C motif chemokine ligand 8 (CXCL8), Toll-like receptor 4 (TLR4), Matrix metalloproteinase 9 (MMP9), Tumor necrosis factor receptor superfamily member 1A (TNFRSF1A), Prostaglandin-endoperoxide synthase 2 (PTGS2), Signal transducer and activator of transcription 1 (STAT1), B-cell lymphoma 2 (BCL2), and RELA proto-oncogene, NF-κB subunit (RELA). ROC curve analysis demonstrated that the combination of these eight feature genes exhibited the highest diagnostic potential. Among them, CXCL8 was the most valuable gene. qRT-PCR analysis showed that CXCL8 mRNA expression was significantly lower in the case group compared to the control group (P < 0.01). The results suggest that ERS is involved in physiological processes such as inflammation and neuronal apoptosis in epilepsy. This provides a bioinformatics evidence for exploring the biological functions and pathology of ERS in epilepsy, as well as serving as a reference for clinical diagnosis and potential therapeutic targets.

Indexed as

Endoplasmic Reticulum StressEpilepsyDatabases, GeneticGene Expression ProfilingGene Regulatory NetworksHumansProtein Interaction MapsProto-Oncogene MasReproducibility of ResultsROC CurveMAS1 protein, humanProto-Oncogene MasBioinformaticsDEGsEndoplasmic reticulum stressEpilepsy

Identifiers

PMID40413658
PMCPMC12104130

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.