Evidence map›Paper›PMID 40414857›Full record

ArticleBMC veterinary research2025

Genetic variation in NSP4 of type 1 porcine reproductive and respiratory syndrome virus in China.

Huiyang Sha, Xiaolin Lan, Zhiyu Yang, Chen Lv, Hang Zhang, Qin Luo, Yajie Zheng, Gan Li, Weili Kong, Liangzong Huang and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Huiyang ShaGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Animal Science and Technology, Foshan University, Foshan, 528225, China.
Xiaolin LanGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Animal Science and Technology, Foshan University, Foshan, 528225, China.
Zhiyu YangGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Animal Science and Technology, Foshan University, Foshan, 528225, China.
Chen LvGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Animal Science and Technology, Foshan University, Foshan, 528225, China.
Hang ZhangGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Animal Science and Technology, Foshan University, Foshan, 528225, China.
Qin LuoGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Animal Science and Technology, Foshan University, Foshan, 528225, China.
Yajie ZhengGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Animal Science and Technology, Foshan University, Foshan, 528225, China.
Gan LiGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Animal Science and Technology, Foshan University, Foshan, 528225, China.
Weili KongGladstone Institutes of Virology and Immunology, University of California, San Francisco, CA, 94158, USA.
Liangzong HuangGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Animal Science and Technology, Foshan University, Foshan, 528225, China.
Jun MaGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Animal Science and Technology, Foshan University, Foshan, 528225, China. majun@fosu.edu.cn.
Mengmeng ZhaoGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Animal Science and Technology, Foshan University, Foshan, 528225, China. mengmengzhao2021@fosu.edu.cn.

Funding

Guangdong Provincial Department of Education's distinctive innovation initiative 2023KTSCX128National Key Research and Development Program Projects 2023YFD1801302National Natural Science Foundation of China 31902279
6 · The paper itself

Abstract

backgroundPorcine reproductive and respiratory syndrome virus (PRRSV) is characterized by a high mutation rate. Although research has largely focused on PRRSV-2, the detection rate of PRRSV-1 in China is increasing. To investigate the prevalence and genetic variation of NSP4, a key gene in the regulation of host immunity, in PRRSV-1 in China, publicly available whole-genome sequence data and NSP4 sequences were obtained for analyses of nucleotide and amino acid homology, substitutions, and phylogenetic relationships.

resultsThe nucleotide homology of NSP4 between 32 PRRSV-1 and 8 PRRSV-2 strains ranged from 57.3 to 62.4%, and the amino acid homology ranged from 58.6 to 64.5%. Among the 32 PRRSV-1 strains, the nucleotide homology of NSP4 ranged from 75.4 to 100.0% and the amino acid homology ranged from 81.3 to 100.0%. Comparisons of NSP4 amino acid sequences of 32 PRRSV-1 and 8 PRRSV-2 strains revealed a deletion at the 83rd amino acid residue in PRRSV-1 and mutations at various amino acid residues. A phylogenetic analysis showed that the PRRSV-1 strains isolated in China clustered into four subgroups of Subtype I Global: Amervac-like, BJEU06-1-like, HKEU16-like, and NMEU09-1-like strains.

conclusionPRRSV-1 NSP4 exhibits similarities to Subtype I Global strains and clusters into four subgroups. Although it differs from PRRSV-2 NSP4 with a deletion in the 83rd amino acid residue, the amino acid residues of the catalytic triad are conserved. These findings have important implications for the development of secure and reliable detection methods as well as new drugs and vaccines and are expected to guide further research on PRRSV-1.

Indexed as

Genetic VariationPorcine Reproductive and Respiratory SyndromePorcine respiratory and reproductive syndrome virusViral Nonstructural ProteinsAmino Acid SequenceAnimalsChinaPhylogenySwineViral Nonstructural ProteinsGenetic variationNSP4Phylogenetic analysisPRRSV-1PRRSV-2

Identifiers

PMID40414857
PMCPMC12105217

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.