Evidence map›Paper›PMID 40417754›Full record

ArticleCurrent pharmaceutical design2025

Investigation into the Mechanisms of Paeoniae Radix Rubra in the Treatment of Venous Thrombosis Using Network Pharmacology, Bioinformatics, and Molecular Docking Techniques.

Shuo Xu, Ajiao Hou, Jiaxu Zhang, Jinhao Xue, Shiwen Gao, Hai Jiang, Liu Yang

Abstract read
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In one paragraph

Article in Current pharmaceutical design, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Shuo XuKey Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin, China.
Ajiao HouKey Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin, China.
Jiaxu ZhangKey Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin, China.
Jinhao XueKey Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin, China.
Shiwen GaoKey Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin, China.
Hai JiangKey Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin, China.
Liu YangKey Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin, China.

Funding

Construction of key disciplines of Chinese medicine processing [2023]National Famous Elderly Traditional Chinese Medicine Experts Inheritance Studio Construction Project 75National Training Program for Traditional Chinese Medicine Characteristic Technology Inheritance Talents [2023]Seventh Batch of National Old Chinese Medicine Experts Academic Experience Inheritance Project 76Traditional Chinese medicine Processing technology inheritance base project and the Graduate Innovative Research Project Foundation of Heilongjiang University of Chinese Medicine 2024yjscx005
6 · The paper itself

Abstract

objectiveThis study investigates the potential targets and mechanisms of Paeoniae Radix Rubra (PRR) in treating Venous Thrombosis (VTE) by employing network pharmacology, bioinformatics analysis, and molecular docking validation.

methodsActive components of PRR were identified via TCMSP. VTE-related genes were screened from GEO datasets, and WGCNA analyzed key modules. A Protein-Protein Interaction (PPI) network was constructed using Cytoscape, followed by immune infiltration analysis. Core targets were functionally annotated via GO and KEGG pathways. Molecular docking and molecular dynamics simulations validated interactions between PRR components and core targets.

resultsA total of 30 active components of PRR and 21 potential targets for the treatment of VTE were identified. From the PPI network, 10 hub genes were screened. KEGG pathway enrichment analysis demonstrated that the target genes were significantly enriched in pathways, such as the cGMP-PKG signaling pathway, B cell receptor signaling pathway, Th1 and Th2 cell differentiation, and IL-17 signaling pathway. Molecular docking results revealed that MAPK1, NFATC1, and SELP all had good affinity with the screened active components. Among them, MAPK1 and beta-sitosterol exhibited the highest binding energy of -8.73 kcal/mol. Molecular dynamics simulation results from RMSD, RMSF, HBond, and SASA analyses indicated that the beta-sitosterol-MAPK1 complex maintained good stability.

conclusionThrough this study, it was found that PRR may act on targets, such as MAPK1 and NFATC1, through components like beta-sitosterol and Stigmasterol. Among them, the complex (beta-sitosterol - MAPK1) may be the key active component that plays a role in treating VTE.

Indexed as

Computational BiologyDrugs, Chinese HerbalMolecular Docking SimulationNetwork PharmacologyPaeoniaVenous ThrombosisHumansMolecular Dynamics SimulationPlant ExtractsProtein Interaction MapsDrugs, Chinese Herbalpeony root extractPlant Extractsbioinformatics analysisimmune cell infiltrationmolecular docking.network pharmacologyPaeoniae Radix Rubra (PRR)Venous thrombosis

Identifiers

PMID40417754

What Socratic holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.