ArticleClinical and experimental dental research2025
Gingival Stem Cell-Conditioned Media and Low-Level Laser Therapy Enhance Periodontal Ligament Stem Cells Function by Upregulating Wnt and TGF-β Pathway Components: An In Vitro Study.
Article in Clinical and experimental dental research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Photobiomodulation Therapy and Wnt/β-Catenin Signaling in Periodontal Ligament Stem Cells: is there a Mechanistic Link Accelerating Orthodontic Tooth Movement?Stem cell reviews and reports · 2026Article
- Article
- Exploring vitamin K2 and B12 as bioactive micronutrients for dentin conditioning in regenerative endodontics procedures.Naunyn-Schmiedeberg's archives of pharmacology · 2026Article
- Use of Laser in Periodontal Tissue Regeneration: A Scoping Review of Clinical and Experimental Evidence.Medicina (Kaunas, Lithuania) · 2025Article
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Authors and funding
5 authors.
Funding
Abstract
objectivesThis study investigated the synergistic effects of gingival stem cell conditioned media (GSC-CM) and low-level laser therapy (LLLT) on stimulating PDLSCs, explicitly focusing on the molecular basis of enhancing the Wnt and TGF-β signaling pathways. MATERIAL AND
methodsPDLSCs were isolated and GSC-CM were prepared and characterized. Five groups were utilized: control, osteogenic induction medium (positive control), GSC-CM, LLLT (980 nm, 1.5 J/cm²), and a combination of GSC-CM with LLLT. Cell viability was assessed using the MTT assay on Days 1, 2, and 3. Osteogenic differentiation of PDLSCs was assessed using mineralization assays employing Alizarin Red staining, and molecular analyses of osteogenic markers (RUNX2, ALP, OCN) and signaling-related genes (CTNNβ1, TGFβ1) conducted via RT‒PCR.
resultsThe findings revealed that the dual application of GSC-CM and LLLT significantly enhanced cell viability and osteogenic differentiation of PDLSCc compared to the effects of the individual treatment modalities. Moreover, the study revealed elevated expression levels of osteogenic markers across all the experimental groups, specially the combination group, which showed the highest levels. Significantly, the combination treatment group displayed superior outcomes in this regard. The results also suggest potential activation of Wnt/β-catenin and TGF-β signaling components.
conclusionsThis study highlights the significant effects of using GSC-CM and LLLT to improve PDLSC survival and osteogenic differentiation.
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