Evidence map›Paper›PMID 40419580›Full record

ArticleScientific reports2025

Osteotropic properties of mesenchymal stromal cells generated in Masquelet's induced membrane technique.

Masao Suzuki, Masanobu Kamitakahara, Wataru Kihara, Cangyou Xie, Hiromu Kato, Yuki Fukawa, Miwako Hamagaki, Kazuhiro Aoki, Satoshi Ichihara, Muneaki Ishijima and 2 more

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In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Masao SuzukiHand Surgery & Trauma Reconstruction Center, Juntendo University Urayasu Hospital, 2-1-1 Tomioka, Urayasu-shi, Chiba, 279-0021, Japan.
Masanobu KamitakaharaGraduate School of Environmental Studies, Tohoku University, 6-6-20 Aoba, Aramaki, Aoba-ku, Sendai, Miyagi, 980-8579, Japan.
Wataru KiharaDepartment of Orthopedics, Faculty of Medicine, Juntendo University, 3-1-3 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
Cangyou XieDepartment of Basic Oral Health Engineering, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8549, Japan.
Hiromu KatoGraduate School of Environmental Studies, Tohoku University, 6-6-20 Aoba, Aramaki, Aoba-ku, Sendai, Miyagi, 980-8579, Japan.
Yuki FukawaDepartment of Oral Pathology, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8549, Japan.
Miwako HamagakiDivision of Surgical Pathology, Institute of Science Tokyo Hospital, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8519, Japan.
Kazuhiro AokiDepartment of Basic Oral Health Engineering, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8549, Japan.
Satoshi IchiharaHand Surgery & Trauma Reconstruction Center, Juntendo University Urayasu Hospital, 2-1-1 Tomioka, Urayasu-shi, Chiba, 279-0021, Japan.
Muneaki IshijimaDepartment of Orthopedics, Faculty of Medicine, Juntendo University, 3-1-3 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
Tohru IkedaDepartment of Oral Pathology, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8549, Japan. tohrupth.mpa@tmd.ac.jp.
Takatoshi OkudaDepartment of Orthopedics, Faculty of Medicine, Juntendo University, 3-1-3 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan. o-kun@juntendo.ac.jp.

Funding

Japan Society for the Promotion of Science 18K09046, 21K09285 and 24K12339
6 · The paper itself

Abstract

Masquelet's induced membrane technique (MIMT) is widely applied two-step surgery of large bone defects. MIMT involves the fibrous encapsulation of a polymethyl methacrylate cement spacer inserted into the bone defect in the primary surgery. Although the biological characteristics of the fibrous membrane have been studied, the postoperative environment of the bone defect encapsulated with the induced membrane has not been investigated. In this study, critical-sized bone defects encapsulated with the induced membrane were filled with porous calcium-deficient bioresorbable hydroxyapatite granules in the secondary surgery. Single-step non-Masquelet conventional surgery (NMCS) was also performed, and the biological characteristics of the stromal tissues induced around the ceramic granules were compared between the two procedures. Compared with NMCS, the stromal tissue induced in MIMT expressed significantly higher levels of CXCL3, RANKL, BMP-2, and BMP-7. Coculture of the primary osteoblast-like cells with the stromal tissue derived from MIMT significantly stimulated the differentiation of primary osteoblast-like cells compared with that derived from NMCS. When hydroxyapatite granules mixed with bone isografts (50% v/v) were implanted, the amount of newly formed bone was significantly larger in MIMT than in NMCS. These results suggest that MIMT induces mesenchymal stromal cells that support bone metabolism and promote bone regeneration.

Indexed as

Bone RegenerationMesenchymal Stem CellsOsteogenesisAnimalsBone CementsCell DifferentiationCells, CulturedCoculture TechniquesDurapatiteHumansOsteoblastsPolymethyl MethacrylateBone CementsDurapatitePolymethyl MethacrylateInduced membraneMasquelet’s techniqueMesenchymal stromal cellsOsteoblastOsteoclast

Identifiers

PMID40419580
PMCPMC12106703

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.