Evidence map›Paper›PMID 40421932›Full record

ArticleJournal of cell science2025

Resolution in super-resolution microscopy - facts, artifacts, technological advancements and biological applications.

Kirti Prakash, David Baddeley, Christian Eggeling, Reto Fiolka, Rainer Heintzmann, Suliana Manley, Aleksandra Radenovic, Hari Shroff, Carlas Smith, Lothar Schermelleh

Abstract read
In one paragraph

Article in Journal of cell science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Review
  8. Article
  9. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Kirti PrakashDelft Center for Systems and Control, Faculty of Mechanical, Maritime, and Materials Engineering, Technische Universiteit Delft, Delft, 2628 CN, The Netherlands.ORCID 0000-0002-0325-9988
David BaddeleyAuckland Bioengineering Institute, University of Auckland, Auckland, 1010, New Zealand.
Christian EggelingInstitute of Applied Optics and Biophysics and Abbe Center of Photonics, Friedrich-Schiller-University Jena, Jena, 07745, Germany.
Reto FiolkaLyda Hill Department of Bioinformatics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.ORCID 0000-0002-4636-5000
Rainer HeintzmannLeibniz Institute of Photonic Technology, Jena, 07743, Germany.ORCID 0000-0002-4950-1936
Suliana ManleyLaboratory of Experimental Biophysics, School of Basic Sciences, Institute of Physics Interfaculty Institute of Bioengineering, EPFL SB-LEB, Lausanne, 1015, Switzerland.ORCID 0000-0002-4755-4778
Aleksandra RadenovicLaboratory of Nanoscale Biology, School of Engineering, Institute of Bioengineering, EPFL STI IBI-STI LBEN, Lausanne, 1015, Switzerland.ORCID 0000-0001-8194-2785
Hari ShroffJanelia Research Campus, Howard Hughes Medical Institute (HHMI), Ashburn, VA 20147, USA.ORCID 0000-0003-3613-8215
Carlas SmithDelft Center for Systems and Control, Faculty of Mechanical, Maritime, and Materials Engineering, Technische Universiteit Delft, Delft, 2628 CN, The Netherlands.ORCID 0000-0003-0591-5093
Lothar SchermellehDepartment of Biochemistry, University of Oxford, Oxford OX1 3QU, UK.ORCID 0000-0002-1612-9699

Funding

University of Oxford
6 · The paper itself

Abstract

Super-resolution microscopy (SRM) has undeniable potential for scientific discovery, yet still presents many challenges that hinder its widespread adoption, including technical trade-offs between resolution, speed and photodamage, as well as limitations in imaging live samples and larger, more complex biological structures. Furthermore, SRM often requires specialized expertise and complex instrumentation, which can deter biologists from fully embracing the technology. In this Perspective, a follow-up to our recent Q&A article, we aim to demystify these challenges by addressing common questions and misconceptions surrounding SRM. Experts offer practical insights into how biologists can maximize the benefits of SRM while navigating issues such as photobleaching, image artifacts and the limitations of existing techniques. We also highlight recent developments in SRM that continue to push the boundaries of resolution. Our goal is to equip researchers with the crucial knowledge they need to harness the full potential of SRM.

Indexed as

MicroscopyAnimalsArtifactsHumansMicroscopy, FluorescenceContrastResolutionSingle-molecule localization microscopyStimulated emission depletionStructured illumination microscopySuper-resolution microscopy

Identifiers

PMID40421932
PMCPMC12148039

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.