ArticleReproduction (Cambridge, England)2025
Male reproductive tract extracellular vesicles display region-specific heterogeneity in mice.
Article in Reproduction (Cambridge, England), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
8 citing papers in PubMed.
- Epididymosome-Supplemented Extender Induces Changes in Morpho-Functional Traits and microRNA Levels of Post-Thaw Sperm and Improves Embryo Developmental Potential.Molecular reproduction and development · 2026Article
- Transcriptome Sequencing and Differential Analysis of Testes in One- and Two-Year-Old Kazakh Horses.Animals : an open access journal from MDPI · 2026Article
- Environmental influences on seminal plasma: Molecular and functional insights.The Journal of reproduction and development · 2026Review
- Article
- NT5C1B Improves Fertility of Boar Spermatozoa by Enhancing Quality and Cryotolerance During Cryopreservation.Animals : an open access journal from MDPI · 2025Article
- Review
- Role of Seminal Exosomes in Reproduction.Journal of cellular physiology · 2025Review
- Comparative Proteomics of Seminal Exosomes Reveals Size-Exclusion Chromatography Outperforms Ultracentrifugation.Biomedicines · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In brief: Male reproductive tract extracellular vesicles play a critical role in regulating sperm quality and male fertility. This study shows that extracellular vesicles from distinct regions of the male reproductive tract differ in their size, abundance and composition. Abstract: As sperm transit the male reproductive tract, they undergo a series of dynamic changes, gaining motility, modifying lipid and protein content and refining their epigenetic composition. Extracellular vesicles are central to this post-testicular maturation and changes in their composition could directly impact male reproductive health, sperm quality and post-fertilisation development. This study aimed to characterise and compare extracellular vesicles isolated from distinct regions of the male reproductive tract. Extracellular vesicles were isolated from adult, male C57BL/6J cauda and caput epididymis (epididymosomes) and seminal vesicle fluid by precipitation and size exclusion chromatography. Isolated vesicles were characterised using nanoparticle tracking analysis, transmission electron microscopy, Western blotting and imaging flow cytometry. Epididymosomes and seminal fluid vesicles ranged from 110.26 to 121.26 nm in diameter, had a concentration of 109 to 1010 particles/cm3 and had a typical round, cup-shaped morphology. The size and concentration of extracellular vesicles from the caput were significantly larger than those from the cauda and seminal fluid. Imaging flow cytometry revealed that all isolated extracellular vesicles expressed CD81 and CD9 tetraspanins; however, CD63 was detected only in caput epididymosomes. Furthermore, there were significantly fewer CD9+ vesicles in seminal fluid EVs compared to epididymosomes. Using a range of bulk- and single-vesicle analytical approaches, we show that different regions of the male reproductive tract display distinct vesicle compositional phenotypes. However, additional studies are warranted to define the significance of this heterogeneity, their roles in regulating male reproductive health and the development of their offspring.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.