ArticleBMC ophthalmology2025
Alterations in ocular fungal microbiota in patients with fungal keratitis: a comparative study from coastal regions of Eastern China.
Article in BMC ophthalmology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- The Ocular Surface Microbiome in Homeostasis and Dysbiosis.Microorganisms · 2025Review
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Authors and funding
6 authors.
Funding
Abstract
objectiveThe understanding of the ocular microbiota, particularly in fungal keratitis (FK), is evolving with advancements in high-throughput sequencing technologies. Traditional culture-based methods may not fully capture the microbial diversity present in keratitis, highlighting the need for more comprehensive approaches to explore microbial dysbiosis in corneal infections. This study aimed to reveal the dysbacteriosis of the ocular fungal microbiome associated with FK.
methodsWe analysed 105 samples, including conjunctival swabs from healthy eyes (HE) and conjunctival swabs (SW), as well as corneal scrapings (SC), from FK eyes. Positive results were observed in 58 samples, and detailed taxonomic categorization was carried out across multiple levels-phylum, class, order, family, and genus-using high-throughput ITS sequencing. Alpha and beta diversity indices were computed, and interaction networks at the genus level were predicted to elucidate changes in microbial communities. The analyses also included assessments of functional groups within the fungal microbiome.
resultsAmong the samples, the HE, SW, and SC groups presented differences in positivity rates and diversity indices. Compared with HE eyes, infected eyes (SW and SC) presented significantly greater Good's coverage estimator and lower Chao1, Shannon, and Simpson diversity indices, indicating reduced species richness and evenness. At multiple taxonomic levels, various taxa were significantly downregulated in the FK eyes. Functional analyses revealed differences, notably, an increase in the number of litter saprotrophs in FK eyes. Ascomycota and Basidiomycota were identified as core phyla in the ocular microbiota interaction network.
conclusionFungal keratitis significantly alters the ocular surface microbiome, which is characterized by decreased microbial richness and evenness. High-throughput sequencing revealed a complex interaction network with significant variability between healthy and infected eyes. Additionally, these findings suggest potential benefits from early and aggressive debridement in managing FK due to its impact on functional microbial groups.
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