Evidence map›Paper›PMID 40427344›Full record

ArticleAnimals : an open access journal from MDPI2025

Estradiol Reverses Ovariectomy-Induced Disruption of Hypothalamic Gene Expression and Behavior via Modulation of Gonadotropin Releasing Hormone and Calcium Signaling Pathways.

Asim Muhammad, Mubashir Muhammad, Xiaohuan Chao, Chunlei Zhang, Jiahao Chen, Huan Yang, Shuhan Liu, Yuan Ding, Ziming Wang, Hongwei Bi and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Animals : an open access journal from MDPI, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Asim MuhammadCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Mubashir MuhammadCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Xiaohuan ChaoCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Chunlei ZhangCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Jiahao ChenCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Huan YangCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Shuhan LiuCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Yuan DingCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Ziming WangCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Hongwei BiCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Wen GuoCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Junhong FanCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Bo ZhouCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.ORCID 0000-0003-2857-8536

Funding

The National Natural Science Foundation of China 32172786
6 · The paper itself

Abstract

Estrogen plays a crucial role in regulating reproductive and neuroendocrine functions, yet the molecular mechanisms underlying its effects on the hypothalamus remain incompletely understood. This study investigates the transcriptional and behavioral changes induced by ovariectomy (OVX) and estradiol (E2) supplementation in female C57BL/6J mice. RNA sequencing was performed to identify differentially expressed genes (DEGs) across control (CK), E2, OVX, and OVX+E2 groups, followed by functional enrichment and pathway analyses. Behavioral assessments, including open field, Y-maze, and elevated plus maze tests, were conducted to evaluate anxiety-like and cognitive behaviors. Results revealed significant alterations in GnRH signaling, neurotransmission, and inflammatory pathways, with key genes such as

Indexed as

calcium signaling pathwayestradiol interventionGnRH signaling pathwayhypothalamic transcriptomicsovariectomyreproductive aging

Identifiers

PMID40427344
PMCPMC12108420

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.