ArticleInternational journal of molecular sciences2025
Rapid and Cost-Effective Diagnostic Blot Assays Based on the Use of Plant-Produced Recombinant Antigens: Lessons Learned from the SARS-CoV-2 RBD Antigen.
Chiara Miele, Dania Ramadan, Leonardo Lupacchini, Carla Marusic, Valeria D'Argenio, Maria Giovanna Valente, Antonella Spila, Gianluca Gessoni, Veronica Alfano, Patrizia Ferroni and 2 more
Abstract read
In one paragraphArticle in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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2 · The registryThe trial behind it
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3 · Its place in the literatureWho cites it
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4 · The recordCorrections and comments
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5 · Who and what moneyAuthors and funding
12 authors.
Chiara MieleDepartment for the Promotion of Human Sciences and Quality of Life, San Raffaele Roma Open University, 00166 Rome, Italy.
Dania RamadanInterInstitutional Multidisciplinary Biobank (BioBIM), IRCCS San Raffaele, 00166 Roma, Italy.
Leonardo LupacchiniLaboratory of Molecular and Cellular Neuroscience, IRCCS San Raffaele, 00166 Rome, Italy.ORCID 0000-0002-9172-7461 Carla MarusicDivision of Biotechnology, ENEA Casaccia Research Center, 00123 Roma, Italy.
Valeria D'ArgenioDepartment for the Promotion of Human Sciences and Quality of Life, San Raffaele Roma Open University, 00166 Rome, Italy.ORCID 0000-0001-9273-3698 Maria Giovanna ValenteInterInstitutional Multidisciplinary Biobank (BioBIM), IRCCS San Raffaele, 00166 Roma, Italy.ORCID 0000-0003-0093-173X Antonella SpilaInterInstitutional Multidisciplinary Biobank (BioBIM), IRCCS San Raffaele, 00166 Roma, Italy.
Gianluca GessoniTransfusion Medicine Department of Venezia, Ospedale dell'Angelo-Mestre, 30173 Venezia, Italy.
Veronica AlfanoInterInstitutional Multidisciplinary Biobank (BioBIM), IRCCS San Raffaele, 00166 Roma, Italy.
Patrizia FerroniDepartment for the Promotion of Human Sciences and Quality of Life, San Raffaele Roma Open University, 00166 Rome, Italy.ORCID 0000-0002-9877-8712 Fiorella GuadagniDepartment for the Promotion of Human Sciences and Quality of Life, San Raffaele Roma Open University, 00166 Rome, Italy.
Funding
Ministero della Salute Ricerca CorrenteMinistero dell'università e della ricerca 5X1000-2021
6 · The paper itselfAbstract
The ongoing demand for reliable, cost-effective, and scalable diagnostic solutions during the COVID-19 pandemic emphasized the need for innovative production platforms. In this study, we present a plant-based molecular farming (PMF) strategy for the production of the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein fused with an Fc region (RBDw-Fc). The RBDw-Fc antigen was transiently expressed in the
Indexed as
Antigens, ViralCOVID-19COVID-19 Serological TestingSARS-CoV-2Spike Glycoprotein, CoronavirusAntibodies, ViralCost-Benefit AnalysisHumansMolecular FarmingNicotianaPlants, Genetically ModifiedRecombinant ProteinsSensitivity and SpecificityAntibodies, ViralAntigens, ViralRecombinant ProteinsSpike Glycoprotein, Coronavirusspike protein, SARS-CoV-2diagnostic testsplant-based molecular farmingrecombinant antigensSARS-CoV-2
Identifiers
PMID40429644
PMCPMC12111317
What Socratic holds
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LicenceCC BY
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