Evidence map›Paper›PMID 40443032›Full record

ArticleMolecular therapy : the journal of the American Society of Gene Therapy2025

Delivery of AAV vectors to the superior olivary complex enables efficient adult cochlear transduction via axonal transport.

Jerusha Naidoo, Yin Ren, Matthew Rocco, Eric Bielefeld, Allison O'Brien, Hsuan-Chih Kuo, Nathan McFarland, Matthew Avenarius, Manpreet Takhar, Geneva Frank and 2 more

Abstract read
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In one paragraph

Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Jerusha NaidooDepartment of Neurological Surgery, Gene Therapy Institute, The Ohio State University, 2255 Kenny Road, Columbus, OH 43210, USA. Electronic address: jerusha.naidoo@osumc.edu.
Yin RenDepartment of Otolaryngology- Head and Neck Surgery, The Ohio State University, 915 Olentangy River Road, Columbus, OH 43212, USA.
Matthew RoccoDepartment of Neurological Surgery, Gene Therapy Institute, The Ohio State University, 2255 Kenny Road, Columbus, OH 43210, USA.
Eric BielefeldDepartment of Speech and Hearing Science, The Ohio State University, 1070 Carmack Road, Columbus, OH 43210, USA.
Allison O'BrienDepartment of Neurological Surgery, Gene Therapy Institute, The Ohio State University, 2255 Kenny Road, Columbus, OH 43210, USA.
Hsuan-Chih KuoDepartment of Otolaryngology- Head and Neck Surgery, The Ohio State University, 915 Olentangy River Road, Columbus, OH 43212, USA.
Nathan McFarlandDepartment of Neurological Surgery, Gene Therapy Institute, The Ohio State University, 2255 Kenny Road, Columbus, OH 43210, USA.
Matthew AvenariusDepartment of Pathology, The Ohio State University, 410 W 10th Avenue, Columbus, OH 43210, USA.
Manpreet TakharDepartment of Neurological Surgery, Gene Therapy Institute, The Ohio State University, 2255 Kenny Road, Columbus, OH 43210, USA.
Geneva FrankDepartment of Neurological Surgery, Gene Therapy Institute, The Ohio State University, 2255 Kenny Road, Columbus, OH 43210, USA.
Victor Van LaarDepartment of Neurological Surgery, Gene Therapy Institute, The Ohio State University, 2255 Kenny Road, Columbus, OH 43210, USA.
Krystof BankiewiczDepartment of Neurological Surgery, Gene Therapy Institute, The Ohio State University, 2255 Kenny Road, Columbus, OH 43210, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Significant breakthroughs have been made in translation of adeno-associated virus (AAV) vectors for hearing disorders targeting auditory hair cells (HCs). In addition to HCs, spiral ganglion neurons (SGNs) are also impacted in a large number of sensorineural hearing loss cases in adults. However when administered directly into the cochlea in rodents aged older than P1-P3, AAV-mediated SGN transduction efficiency decreases dramatically. An efficient gene-delivery method to transduce adult SGNs is needed. Our group has a track record of utilizing axonal transport to transduce brain structures distal from the site of AAV injection. We investigated whether SGNs could be transduced in adult rats following intraparenchymal AAV administration to the olivary complex. Cochlear transduction was observed with the following common AAV serotypes expressing green fluorescent protein: AAV6, AAV9, AAV-Anc80, and AAV-PhP.B. Cochlear transduction was observed with all serotypes, but the cellular tropism and efficiency of gene transfer varied across the cochlear spiral (apex, middle, base) with different AAV serotypes, with some transducing both SGNs and HCs, while others transduced SGNs or HCs exclusively. This study provides proof of concept that AAV delivery to the olivary complex can efficiently deliver transgenes to SGNs in the adult mammalian cochlea.

Indexed as

Axonal TransportCochleaDependovirusGenetic VectorsTransduction, GeneticAnimalsGenetic TherapyGene Transfer TechniquesHair Cells, AuditoryHumansMaleRatsSpiral GanglionAAVadeno-associated virusauditory nervecochleacochlea nucleusgene therapyhair cellshearingolivary complexspiral ganglion neurons

Identifiers

PMID40443032
PMCPMC12432906

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.