Evidence map›Paper›PMID 40443053›Full record

ArticleMolecular oncology2025

Dose-dependent induction of epithelial-mesenchymal transition in 3D melanoma models by non-thermal plasma treatment.

Eline Biscop, Edgar Cardenas De La Hoz, Hanne Verswyvel, Joey De Backer, Ho Wa Lau, Angela Privat-Maldonado, Wim Vanden Berghe, Steve Vanlanduit, Evelien Smits, Annemie Bogaerts and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular oncology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Eline BiscopPLASMANT, Department of Chemistry, University of Antwerp, Belgium.ORCID 0000-0002-2123-8910
Edgar Cardenas De La HozIndustrial Vision Lab, Department of Applied Engineering, University of Antwerp, Belgium.
Hanne VerswyvelPLASMANT, Department of Chemistry, University of Antwerp, Belgium.
Joey De BackerCell Death Signaling, Epigenetics Lab - Integrated Personalized & Precision Oncology Network (IPPON University of Antwerp), Belgium.
Ho Wa LauCenter for Oncological Research - Integrated Personalized & Precision Oncology Network (IPPON), University of Antwerp, Belgium.
Angela Privat-MaldonadoPLASMANT, Department of Chemistry, University of Antwerp, Belgium.
Wim Vanden BergheCell Death Signaling, Epigenetics Lab - Integrated Personalized & Precision Oncology Network (IPPON University of Antwerp), Belgium.
Steve VanlanduitIndustrial Vision Lab, Department of Applied Engineering, University of Antwerp, Belgium.
Evelien SmitsCenter for Oncological Research - Integrated Personalized & Precision Oncology Network (IPPON), University of Antwerp, Belgium.
Annemie BogaertsPLASMANT, Department of Chemistry, University of Antwerp, Belgium.
Abraham LinPLASMANT, Department of Chemistry, University of Antwerp, Belgium.ORCID 0000-0003-0097-3323

Funding

Fonds Wetenschappelijk Onderzoek 12S9221NFonds Wetenschappelijk Onderzoek 1S67621NFonds Wetenschappelijk Onderzoek G033020NFonds Wetenschappelijk Onderzoek G044420N
6 · The paper itself

Abstract

Despite the promising results of non-thermal plasma (NTP) devices for cancer therapy, the potential adverse effects of NTP irradiation have remained unexplored, including the effects on epithelial-mesenchymal transition (EMT) and subsequent cancer metastasis. In this study, we investigate NTP-induced EMT initiation and progression. A microsecond-pulsed dielectric barrier discharge plasma device was used for NTP treatment, and chicken chorioallantoic membrane (CAM) and spheroids were used as 3D tumor models. NTP treatment reduced tumor volume in the CAM model, but a shift towards a mesenchymal-like phenotype was also measured in melanoma tumors via analysis of the six EMT biomarkers, though changes in cancer cell migration to other organs were not significant. In the spheroid model, molecular analysis also indicated an EMT response following NTP treatment, and enhanced cell migration was measured in one cell line. EMT induction with NTP was dose-dependent and transient; high NTP treatments caused significant EMT response and enhanced migration, but low NTP doses did not. Our findings highlight the important role of NTP parameters for cancer treatment and consequential EMT responses. The insights obtained here further build the foundation for clinical optimization, harnessing the cancer-killing potential of NTP while safeguarding against undesirable EMT-related outcomes.

Indexed as

Epithelial-Mesenchymal TransitionMelanomaPlasma GasesAnimalsCell Line, TumorCell MovementChick EmbryoChorioallantoic MembraneHumansSpheroids, CellularPlasma Gasesdielectric barrier dischargeepithelial‐mesenchymal transitionmelanomaplasma medicineplasma oncology

Identifiers

PMID40443053
PMCPMC12591304

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.